Composite Scaffolds Based on Intestinal Extracellular Matrices and Oxidized Polyvinyl Alcohol: A Preliminary Study for a New Regenerative Approach in Short Bowel Syndrome
Joint Authors
Parnigotto, Pier Paolo
Porzionato, Andrea
De Caro, Raffaele
Macchi, Veronica
Grandi, Francesca
Barbon, Silvia
Rambaldo, Anna
Contran, Martina
Fascetti Leon, Francesco
Stocco, Elena
Gamba, PierGiorgio
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-27
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Pediatric Short Bowel Syndrome is a rare malabsorption disease occurring because of massive surgical resections of the small intestine.
To date, the issues related to current strategies including intestinal transplantation prompted the attention towards tissue engineering (TE).
This work aimed to develop and compare two composite scaffolds for intestinal TE consisting of a novel hydrogel, that is, oxidized polyvinyl alcohol (OxPVA), cross-linked with decellularized intestinal wall as a whole (wW/OxPVA) or homogenized (hW/OxPVA).
A characterization of the supports was performed by histology and Scanning Electron Microscopy and their interaction with adipose mesenchymal stem cells occurred by MTT assay.
Finally, the scaffolds were implanted in the omentum of Sprague Dawley rats for 4 weeks prior to being processed by histology and immunohistochemistry (CD3; F4/80; Ki-67; desmin; α-SMA; MNF116).
In vitro studies proved the effectiveness of the decellularization, highlighting the features of the matrices; moreover, both supports promoted cell adhesion/proliferation even if the wW/OxPVA ones were more effective (p<0.01).
Analysis of explants showed a continuous and relatively organized tissue wall around the supports with a connective appearance, such as myofibroblastic features, smooth muscle, and epithelial cells.
Both scaffolds, albeit with some difference, were promising; nevertheless, further analysis will be necessary.
American Psychological Association (APA)
Grandi, Francesca& Stocco, Elena& Barbon, Silvia& Rambaldo, Anna& Contran, Martina& Fascetti Leon, Francesco…[et al.]. 2018. Composite Scaffolds Based on Intestinal Extracellular Matrices and Oxidized Polyvinyl Alcohol: A Preliminary Study for a New Regenerative Approach in Short Bowel Syndrome. BioMed Research International،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1128722
Modern Language Association (MLA)
Grandi, Francesca…[et al.]. Composite Scaffolds Based on Intestinal Extracellular Matrices and Oxidized Polyvinyl Alcohol: A Preliminary Study for a New Regenerative Approach in Short Bowel Syndrome. BioMed Research International No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1128722
American Medical Association (AMA)
Grandi, Francesca& Stocco, Elena& Barbon, Silvia& Rambaldo, Anna& Contran, Martina& Fascetti Leon, Francesco…[et al.]. Composite Scaffolds Based on Intestinal Extracellular Matrices and Oxidized Polyvinyl Alcohol: A Preliminary Study for a New Regenerative Approach in Short Bowel Syndrome. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1128722
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128722