Negative Regulation of PTEN by MicroRNA-221 and Its Association with Drug Resistance and Cellular Senescence in Lung Cancer Cells
Joint Authors
Wang, Ning
Zhu, Chen
Xu, Ye
Qian, Wenliang
Zheng, Min
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
Chemotherapy is the routine method for treating many cancers, but long-term treatment may result in developing resistance to the drugs.
The aim of this study was to identify whether noncoding RNAs play a role in drug resistance and how they affect drug resistance.
Materials and Methods.
The expression levels of miR-221 in different lung cancer cell lines H226, H1299, and A549 were measured.
H1299 and A549 cell lines were transfected to overexpress and downexpress miR-221, and cell viability and cell senescence were determined.
The PTEN/Akt pathway was then examined by real-time polymerase chain reaction and Western blot analysis.
Results.
MiR-221 together with proteins MDR1 and ABCG2 was upregulated in Cisplatin-resistant A549 lung cancer cells.
Anti-miR-221 inhibits proliferation and induces senescence in lung cancer cells.
PTEN/Akt pathway axis was identified as a target of drug resistance induced by miR-221.
Conclusion.
Our results revealed that miR-221 is an important regulator for chemotherapy sensitivity and showed miR-221 as a potential target for drug sensitization.
American Psychological Association (APA)
Wang, Ning& Zhu, Chen& Xu, Ye& Qian, Wenliang& Zheng, Min. 2018. Negative Regulation of PTEN by MicroRNA-221 and Its Association with Drug Resistance and Cellular Senescence in Lung Cancer Cells. BioMed Research International،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1128770
Modern Language Association (MLA)
Wang, Ning…[et al.]. Negative Regulation of PTEN by MicroRNA-221 and Its Association with Drug Resistance and Cellular Senescence in Lung Cancer Cells. BioMed Research International No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1128770
American Medical Association (AMA)
Wang, Ning& Zhu, Chen& Xu, Ye& Qian, Wenliang& Zheng, Min. Negative Regulation of PTEN by MicroRNA-221 and Its Association with Drug Resistance and Cellular Senescence in Lung Cancer Cells. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1128770
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128770