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A Novel UDP-Glycosyltransferase of Rhodiola crenulata Converts Tyrosol to Specifically Produce Icariside D2
Joint Authors
Liao, Zhihua
Qiu, Fei
Zeng, Junlan
Gu, Li
Wang, Bangjun
Lan, Xiaozhong
Chen, Min
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Rhodiola crenulata is a Tibetan native herbal plant belonging to the family of Crassulaceae, which produces the pharmaceutical icariside D2 with the activities of inhibiting angiotensin-converting enzyme and killing leukemia cancer cells.
In this study, we functionally characterized a novel UDP-glycosyltransferase (RcUGT1) that converted tyrosol to specifically produce icariside D2 from R.
crenulata at molecular and biochemical levels.
RcUGT1 was highly expressed in flowers and roots, while the icariside D2 content was much higher in stems than that in other organs, suggesting the potential translocation of icariside D2 from flowers and roots to stems.
The high production of icariside D2 in stems provided a reasonable suggestion to farmers to harvest stems instead of roots for icariside D2 production.
Enzymatic assays of recombinant RcUGT1 indicated that it converted tyrosol to specifically form icariside D2, with the values of Km 0.97±0.10 mM, Vmax 286±8.26 pKat/mg, Kcat 0.01552 s−1, and Kcat/Km 159.55 s−1 M−1.
Functional identification of RcUGT1 facilitated the icariside D2 production through metabolic engineering in plants or synthetic biology in microbes.
American Psychological Association (APA)
Liao, Zhihua& Qiu, Fei& Zeng, Junlan& Gu, Li& Wang, Bangjun& Lan, Xiaozhong…[et al.]. 2018. A Novel UDP-Glycosyltransferase of Rhodiola crenulata Converts Tyrosol to Specifically Produce Icariside D2. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1128840
Modern Language Association (MLA)
Liao, Zhihua…[et al.]. A Novel UDP-Glycosyltransferase of Rhodiola crenulata Converts Tyrosol to Specifically Produce Icariside D2. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1128840
American Medical Association (AMA)
Liao, Zhihua& Qiu, Fei& Zeng, Junlan& Gu, Li& Wang, Bangjun& Lan, Xiaozhong…[et al.]. A Novel UDP-Glycosyltransferase of Rhodiola crenulata Converts Tyrosol to Specifically Produce Icariside D2. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1128840
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128840