Indole-3-Carbinol Induces Apoptosis in Human Osteosarcoma MG-63 and U2OS Cells
Joint Authors
Lee, Chang Min
Nam, Myeong Jin
Park, See-Hyoung
Lee, Jongsung
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-29
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
This study was focused on investigating the anticancer potential of indole-3-carbinol (I3C) against osteosarcoma MG-63 and U2OS cells.
A wound healing assay indicated that IC3 inhibited migration of MG-63 and U2OS cells.
MTT, WST-1, and colony formation assays revealed that treatment of MG-63 and U2OS cells with I3C decreased cell viability.
Fluorescence-activated cell sorting (FACS) analysis showed that I3C induced apoptosis in a dose- and time-dependent manner in MG-63 and U2OS cells.
Moreover, via terminal deoxynucleotidyl transferase- (TdT-) mediated dUTP-biotin nick-end labeling (TUNEL) assay, we detected that I3C induced DNA fragmentation.
Western blotting demonstrated that activated forms of caspase-3, caspase-7, and caspase-9, as well as poly (ADP-ribose) polymerase (PARP) were increased in MG-63 and U2OS cells, following treatment with I3C.
Furthermore, protein expression levels of FOXO3, Bax, and Bim extra-large form were increased while those of Akt, JNK, p38, phosphorylated ERK, and Bcl-xL were decreased by I3C treatment in MG-63 and U2OS cells.
Thus, the study indicates that I3C may induce apoptosis in human osteosarcoma MG-63 and U2OS cells via the activation of apoptotic signaling pathways by FOXO3.
American Psychological Association (APA)
Lee, Chang Min& Lee, Jongsung& Nam, Myeong Jin& Park, See-Hyoung. 2018. Indole-3-Carbinol Induces Apoptosis in Human Osteosarcoma MG-63 and U2OS Cells. BioMed Research International،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1128841
Modern Language Association (MLA)
Lee, Chang Min…[et al.]. Indole-3-Carbinol Induces Apoptosis in Human Osteosarcoma MG-63 and U2OS Cells. BioMed Research International No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1128841
American Medical Association (AMA)
Lee, Chang Min& Lee, Jongsung& Nam, Myeong Jin& Park, See-Hyoung. Indole-3-Carbinol Induces Apoptosis in Human Osteosarcoma MG-63 and U2OS Cells. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1128841
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128841