Optimization of Cultural Conditions for Bacillus megaterium Cultured in Agaricus bisporus Industrial Wastewater
Joint Authors
Huang, Jiafu
Zhang, Danfeng
Ou, Yixin
Zhang, Guoguang
Zheng, Linhua
Lin, Lizhuan
Ye, Xiaomei
Zhu, Xiaofeng
Pan, Yutian
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The aim of this study was to optimize the cultural conditions for Bacillus megaterium using Agaricus bisporus industrial wastewater as nature culture through response surface methodology.
In our present study, we analyzed the total number of living B.
megaterium in the fermentation broth using multispectral imaging flow cytometry.
Plackett-Burman design was applied to evaluate the effects of six variables, namely, initial pH, industrial wastewater solubility, rotating speed, culture temperature, inoculum size, and loading volume.
Loading volume, initial pH, and culture temperature were found to influence the biomass of B.
megaterium significantly and were further optimized by Box-Behnken design.
After verification test, the optimum fermentation conditions of B.
megaterium using the A.
bisporus processing wastewater as nature culture media were obtained as follows: initial pH of 7.4, culture temperature of 25°C, loading volume of 40 mL/250 mL, culture time of 24 h, industrial wastewater solubility of 1%, rotating speed of 200 rpm, and inoculum size of 8%.
The predicted optimum model’s value was 8.88 × 108 Obj/mL and the average experimental value was 9.03 ± 0.02 × 108 Obj/mL, which met the national microbial fertilizers’ standard.
Furthermore, the field experiment results showed that the fermentation broth of B.
megaterium could significantly improve the yield of Spinacia oleracea L.
American Psychological Association (APA)
Huang, Jiafu& Zhang, Danfeng& Ou, Yixin& Zhang, Guoguang& Zheng, Linhua& Lin, Lizhuan…[et al.]. 2018. Optimization of Cultural Conditions for Bacillus megaterium Cultured in Agaricus bisporus Industrial Wastewater. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1128893
Modern Language Association (MLA)
Huang, Jiafu…[et al.]. Optimization of Cultural Conditions for Bacillus megaterium Cultured in Agaricus bisporus Industrial Wastewater. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1128893
American Medical Association (AMA)
Huang, Jiafu& Zhang, Danfeng& Ou, Yixin& Zhang, Guoguang& Zheng, Linhua& Lin, Lizhuan…[et al.]. Optimization of Cultural Conditions for Bacillus megaterium Cultured in Agaricus bisporus Industrial Wastewater. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1128893
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128893