Optimization of Subcritical Water Extraction (SWE)‎ of Lipid and Eicosapentaenoic Acid (EPA)‎ from Nannochloropsis gaditana

Abstract EN

Microalgae are a promising source of omega-3.

The purpose of this study was to extract lipid with a relatively high content of eicosapentaenoic acid (EPA) from Nannochloropsis gaditana using subcritical water extraction (SWE).

The effects of different temperatures (156.1-273.9°C), extraction times (6.6-23.4 minutes), and biomass loadings (33-117 g algae/L) on the extraction yield were studied.

From the optimization study using central composite design (CCD), quadratic models generated for lipid yield and EPA composition were considered to be significant models (p < 0.05).

The predictive equations were also formed for lipid yield and EPA composition.

The predicted optimum lipid yield and EPA composition at 236.54°C, 13.95 minutes, and 60.50 g algae/L were 18.278 wt% of total biomass and 14.036 wt% of total fatty acid methyl ester (FAME), respectively.