Pyrethroid Acaricide Resistance Is Proportional to P-450 Cytochrome Oxidase Expression in the Cattle Tick Rhipicephalus microplus
Joint Authors
Cossío-Bayúgar, Raquel
Martínez-Ibañez, Francisco
Aguilar-Díaz, Hugo
Miranda-Miranda, Estefan
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-04
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The goal of the present study was to assess the gene expression of xenobiotic metabolizing enzymes (XMEs) Cytochrome P-450 (CYP) and carboxylesterase (CE) related to detoxification of synthetic pyrethroids, plus acetylcholinesterase (AChE), in field isolates of acaricide-resistant Rhipicephalus microplus.
The XMEs expression levels were assessed by mRNA measurement using quantitative reverse transcription PCR.
The XME expression levels of field-isolated acaricide-resistant ticks were compared against acaricide-susceptible reference ticks used in this study as a gene expression baseline and represented as relative expression units (REU).
Field isolates were subjected to toxicological bioassays and determined resistant to all the Pyr acaricides (Pyr), whereas most of them were found susceptible to organophosphorous acaricides (OP), with the exception of three isolates, which exhibited moderate resistance to Diazinon.
Significantly higher levels of CYP were detected in pyrethroid-resistance ticks when compared to Su ticks (P<0.01).
A linear regression analysis showed that pyrethroid acaricide resistance levels of R.
microplus were proportional to the CYP expression levels (correlation coefficient (R):0.85; P<0.05).
Analysis on CE expression levels showed only one isolate resistant to Pyr and OP with a statistically significant increase (P<0.01).
AChE expression levels showed statistically significant (P<0.01) subexpression in all tick isolates when compared to the susceptible reference.
Our results suggest that pyrethroid acaricide resistance in the cattle tick may be diagnosed by measuring the CYP expression levels using quantitative PCR.
American Psychological Association (APA)
Cossío-Bayúgar, Raquel& Martínez-Ibañez, Francisco& Aguilar-Díaz, Hugo& Miranda-Miranda, Estefan. 2018. Pyrethroid Acaricide Resistance Is Proportional to P-450 Cytochrome Oxidase Expression in the Cattle Tick Rhipicephalus microplus. BioMed Research International،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1129034
Modern Language Association (MLA)
Cossío-Bayúgar, Raquel…[et al.]. Pyrethroid Acaricide Resistance Is Proportional to P-450 Cytochrome Oxidase Expression in the Cattle Tick Rhipicephalus microplus. BioMed Research International No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1129034
American Medical Association (AMA)
Cossío-Bayúgar, Raquel& Martínez-Ibañez, Francisco& Aguilar-Díaz, Hugo& Miranda-Miranda, Estefan. Pyrethroid Acaricide Resistance Is Proportional to P-450 Cytochrome Oxidase Expression in the Cattle Tick Rhipicephalus microplus. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1129034
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129034