The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study
Joint Authors
Mulla, Tabassum
Patil, Sushama
Sistla, Srinivas
Jadhav, Jyoti
Source
Biochemistry Research International
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Yam tyrosinase has become an economically essential enzyme due to its ease of purification and abundant availability of yam tubers.
However, an efficient biochemical and biophysical characterization of yam tyrosinase has not been reported.
In the present study, the interaction of yam (Amorphophallus paeoniifolius) tyrosinase was studied with molecules such as crocin (Crocus sativus), hydroquinone, and kojic acid.
Surface plasmon resonance (SPR), fluorescence spectroscopy, and circular dichroism techniques were employed to determine the binding affinities and the changes in secondary and tertiary structures of yam tyrosinase in the presence of four relevant small molecules.
Hydroquinone and crocin exhibited very low binding affinities of 0.24 M and 0.0017 M.
Due to their apparent weak interactions, competition experiments were used to determine more precisely the binding affinities.
Structure-function interrelationships can be correlated in great detail by this study, and the results can be compared with other available tyrosinases.
American Psychological Association (APA)
Mulla, Tabassum& Patil, Sushama& Sistla, Srinivas& Jadhav, Jyoti. 2019. The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study. Biochemistry Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1129080
Modern Language Association (MLA)
Mulla, Tabassum…[et al.]. The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study. Biochemistry Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1129080
American Medical Association (AMA)
Mulla, Tabassum& Patil, Sushama& Sistla, Srinivas& Jadhav, Jyoti. The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study. Biochemistry Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1129080
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129080