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Ischemic Preconditioning Promotes Autophagy and Alleviates Renal IschemiaReperfusion Injury
Joint Authors
Xie, Ying
Xiao, Jing
Fu, Chensheng
Zhang, Zhenxing
Ye, Zhibin
Zhang, Xiaoli
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Autophagy is important for cellular survival during renal ischemia/reperfusion (I/R) injury.
Ischemic preconditioning (IPC) has a strong renoprotective effect during renal I/R.
Our study here aimed to explore the effect of IPC on autophagy during renal I/R injury.
Rats were subjected to unilateral renal ischemia with or without prior IPC.
Hypoxia/reoxygenation (H/R) injury was induced in HK-2 cells with or without prior hypoxic preconditioning (HPC).
Autophagy and apoptosis were detected after reperfusion or reoxygenation for different time.
The results showed that the levels of LC3II, Beclin-1, SQSTM1/p62, and cleaved caspase-3 were altered in a time-dependent manner during renal I/R.
IPC further induced autophagy as indicated by increased levels of LC3II and Beclin-1, decreased level of SQSTM1/p62, and accumulation of autophagosomes compared to I/R groups at corresponding reperfusion time.
In addition, IPC reduced the expression of cleaved caspase-3 and alleviated renal cell injury, as evaluated by the levels of serum creatinine (Scr), neutrophil gelatinase-associated lipocalin (NGAL), and kidney injury molecule-1 (KIM-1) in renal tissues.
In conclusion, autophagy and apoptosis are dynamically altered during renal I/R.
IPC protects against renal I/R injury and upregulates autophagic flux, thus increasing the possibility for a novel therapy to alleviate I/R-induced acute kidney injury (AKI).
American Psychological Association (APA)
Xie, Ying& Xiao, Jing& Fu, Chensheng& Zhang, Zhenxing& Ye, Zhibin& Zhang, Xiaoli. 2018. Ischemic Preconditioning Promotes Autophagy and Alleviates Renal IschemiaReperfusion Injury. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1129086
Modern Language Association (MLA)
Xie, Ying…[et al.]. Ischemic Preconditioning Promotes Autophagy and Alleviates Renal IschemiaReperfusion Injury. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1129086
American Medical Association (AMA)
Xie, Ying& Xiao, Jing& Fu, Chensheng& Zhang, Zhenxing& Ye, Zhibin& Zhang, Xiaoli. Ischemic Preconditioning Promotes Autophagy and Alleviates Renal IschemiaReperfusion Injury. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1129086
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129086