Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay
Joint Authors
Xu, Min
Jiao, Yue-Ying
Fu, Yuan-Hui
Jiang, Nan
Zheng, Yuan-Bo
Yan, Yi-Fei
Zhang, Mei
Zheng, Yan-Peng
Zhu, Wu-Yang
Peng, Xiang-Lei
He, Jin-Sheng
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Human respiratory syncytial virus (RSV) is the single most important cause of lower respiratory tract disease in infants and young children and a major viral agent responsible for respiratory tract disease in immunosuppressed individuals and the elderly, but no vaccines and antiviral drugs are available.
Herein the recombinant RSV (rRSV) encoding enhanced green fluorescence protein (EGFP, rRSV-EGFP) was constructed and the potential for screening anti-RSV drugs was investigated.
The recombinant plasmid of pBRATm-rRSV-EGFP, containing T7 transcription cassette composed of T7 promoter, RSV antigenomic cDNA with EGFP gene, HDV ribozyme (δ), and T7 terminator in the order of 5′ to 3′, was constructed and cotransfected into BHK/T7-9 cells together with helper plasmids encoding N, P, L, and M2-1 gene, respectively.
The rescued rRSV-EGFP was confirmed by increasing expression of EGFP over blind passages and by RT-PCR.
rRSV-EGFP was comparable to the other two recombinant RSVs encoding red fluorescent protein (RFP, rRSV-RFP) or luciferase (Luc, rRSV-Luc) in the growth kinetic, and there was a difference in sensitivity between them for screening anti-RSV agents based on infection of HEp-2 cells.
The EGFP-encoding rRSV has been constructed and rescued successfully and has the potential for high-throughput anti-RSV drug screening in vitro.
American Psychological Association (APA)
Xu, Min& Jiao, Yue-Ying& Fu, Yuan-Hui& Jiang, Nan& Zheng, Yuan-Bo& Yan, Yi-Fei…[et al.]. 2018. Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1129145
Modern Language Association (MLA)
Xu, Min…[et al.]. Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1129145
American Medical Association (AMA)
Xu, Min& Jiao, Yue-Ying& Fu, Yuan-Hui& Jiang, Nan& Zheng, Yuan-Bo& Yan, Yi-Fei…[et al.]. Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1129145
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129145