Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay

Joint Authors

Xu, Min
Jiao, Yue-Ying
Fu, Yuan-Hui
Jiang, Nan
Zheng, Yuan-Bo
Yan, Yi-Fei
Zhang, Mei
Zheng, Yan-Peng
Zhu, Wu-Yang
Peng, Xiang-Lei
He, Jin-Sheng

Source

BioMed Research International

Issue

Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2018-01-15

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Medicine

Abstract EN

Human respiratory syncytial virus (RSV) is the single most important cause of lower respiratory tract disease in infants and young children and a major viral agent responsible for respiratory tract disease in immunosuppressed individuals and the elderly, but no vaccines and antiviral drugs are available.

Herein the recombinant RSV (rRSV) encoding enhanced green fluorescence protein (EGFP, rRSV-EGFP) was constructed and the potential for screening anti-RSV drugs was investigated.

The recombinant plasmid of pBRATm-rRSV-EGFP, containing T7 transcription cassette composed of T7 promoter, RSV antigenomic cDNA with EGFP gene, HDV ribozyme (δ), and T7 terminator in the order of 5′ to 3′, was constructed and cotransfected into BHK/T7-9 cells together with helper plasmids encoding N, P, L, and M2-1 gene, respectively.

The rescued rRSV-EGFP was confirmed by increasing expression of EGFP over blind passages and by RT-PCR.

rRSV-EGFP was comparable to the other two recombinant RSVs encoding red fluorescent protein (RFP, rRSV-RFP) or luciferase (Luc, rRSV-Luc) in the growth kinetic, and there was a difference in sensitivity between them for screening anti-RSV agents based on infection of HEp-2 cells.

The EGFP-encoding rRSV has been constructed and rescued successfully and has the potential for high-throughput anti-RSV drug screening in vitro.

American Psychological Association (APA)

Xu, Min& Jiao, Yue-Ying& Fu, Yuan-Hui& Jiang, Nan& Zheng, Yuan-Bo& Yan, Yi-Fei…[et al.]. 2018. Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1129145

Modern Language Association (MLA)

Xu, Min…[et al.]. Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1129145

American Medical Association (AMA)

Xu, Min& Jiao, Yue-Ying& Fu, Yuan-Hui& Jiang, Nan& Zheng, Yuan-Bo& Yan, Yi-Fei…[et al.]. Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1129145

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1129145