Involvement of P2X7 Receptor in Proliferation and Migration of Human Glioma Cells
Joint Authors
Xie, Yuting
Ji, Min
Ji, Zhenhua
Guan, Yu
Zhang, Yujian
Cho, Kin-Sang
You, Yongping
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-09
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Previous studies have demonstrated that activation of P2X7 receptors (P2X7R) results in the proliferation and migration of some types of tumor.
Here, we asked whether and how the activated P2X7R contribute to proliferation and migration of human glioma cells.
Results showed that the number of P2X7R positive cells was increasing with grade of tumor.
In U87 and U251 human glioma cell lines, both expressed P2X7R and the expression was enhanced by 3′-O-(4-benzoylbenzoyl) ATP (BzATP), the agonist of P2X7R, and siRNA.
Our results also showed that 10 μM BzATP was sufficient to induce the proliferation of glioma cell significantly, while the cell proliferation reached the peak with 100 μM BzATP.
Also, the migration of U87 and U251 cells was significantly increased upon BzATP treatment.
However, the number of apoptotic cells of U87 and U251 was not significantly changed by BzATP.
In addition, the expression of ERK, p-ERK, and proliferating cell nuclear antigen (PCNA) protein was increased in BzATP-treated U87 and U251 glioma cells.
PD98059, an inhibitor of the MEK/ERK pathway, blocked the increased proliferation and migration of glioma cells activated by BzATP.
These results suggest that ERK pathway is involved in the proliferation and migration of glioma cells induced by P2X7R activation.
American Psychological Association (APA)
Ji, Zhenhua& Xie, Yuting& Guan, Yu& Zhang, Yujian& Cho, Kin-Sang& Ji, Min…[et al.]. 2018. Involvement of P2X7 Receptor in Proliferation and Migration of Human Glioma Cells. BioMed Research International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1129238
Modern Language Association (MLA)
Ji, Zhenhua…[et al.]. Involvement of P2X7 Receptor in Proliferation and Migration of Human Glioma Cells. BioMed Research International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1129238
American Medical Association (AMA)
Ji, Zhenhua& Xie, Yuting& Guan, Yu& Zhang, Yujian& Cho, Kin-Sang& Ji, Min…[et al.]. Involvement of P2X7 Receptor in Proliferation and Migration of Human Glioma Cells. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1129238
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129238