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Resveratrol Inhibits the TGF-β1-Induced Proliferation of Cardiac Fibroblasts and Collagen Secretion by Downregulating miR-17 in Rat
Joint Authors
Zhang, Yao
Lu, Yuan
Ong’achwa, Machuki Jeremiah
Ge, Liqi
Qian, Yun
Chen, Lei
Hu, Xiaoqin
Li, Fei
Wei, Hui
Zhang, Chaoqun
Li, Chengzong
Wang, Zhirong
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Myocardial fibrosis (MF) can cause heart remodeling and it is an independent risk factor for malignant arrhythmias, sudden cardiac death, and other malignant cardiovascular events.
It is often characterized by myocardial interstitial collagen deposition and hyperproliferation of cardiac fibroblasts (CFs).
The transforming growth factor-β1 (TGF-β1) is the most influential profibrogenic factor.
Resveratrol (RSV) is an active polyphenol substance that inhibits myocardial fibrosis.
The mechanism of RSV-mediated inhibition of the proliferation of CFs at the microRNA level is not fully understood.
We used TGF-β1 to induce CFs proliferation to simulate the pathogenesis of myocardial fibrosis.
Neonatal rat CFs were treated with TGF-β1 in the presence or absence of resveratrol.
Cell proliferation was measured using the CCK-8 and EdU assay.
Collagen secretion was measured using hydroxyproline kit.
Further, qPCR analysis was performed to determine microRNA levels after TGF-β1 or resveratrol treatment.
To identify the target gene for miR-17, miR-17 was overexpressed or silenced, and the mRNA and protein levels of Smad7 were assessed.
The effects of miR-17 silencing or Smad7 overexpression on cell proliferation and collagen secretion were also examined.
Resveratrol treatment significantly decreased the TGF-β1-induced CF proliferation and collagen secretion.
Resveratrol also decreased the levels of miR-17, miR-34a, and miR-181a in TGF-β1-treated CFs.
Overexpression of miR-17 decreased the Smad7 mRNA and protein levels while silencing miR-17 increased them.
Additionally, silencing miR-17 or overexpressing Smad7 decreased the TGF-β1-induced CFs proliferation and collagen secretion.
In conclusion, resveratrol inhibits TGF-β1-induced CFs proliferation and collagen secretion.
This inhibitory effect of resveratrol is orchestrated by the downregulation of miR-17 and the regulation of Smad7.
American Psychological Association (APA)
Zhang, Yao& Lu, Yuan& Ong’achwa, Machuki Jeremiah& Ge, Liqi& Qian, Yun& Chen, Lei…[et al.]. 2018. Resveratrol Inhibits the TGF-β1-Induced Proliferation of Cardiac Fibroblasts and Collagen Secretion by Downregulating miR-17 in Rat. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1129329
Modern Language Association (MLA)
Zhang, Yao…[et al.]. Resveratrol Inhibits the TGF-β1-Induced Proliferation of Cardiac Fibroblasts and Collagen Secretion by Downregulating miR-17 in Rat. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1129329
American Medical Association (AMA)
Zhang, Yao& Lu, Yuan& Ong’achwa, Machuki Jeremiah& Ge, Liqi& Qian, Yun& Chen, Lei…[et al.]. Resveratrol Inhibits the TGF-β1-Induced Proliferation of Cardiac Fibroblasts and Collagen Secretion by Downregulating miR-17 in Rat. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1129329
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129329