Phosphoprotein Contributes to the Thermostability of Newcastle Disease Virus
Joint Authors
Cong, Feng
Zhao, Yang
Liu, Huairan
Wu, Wei
Zhao, Ran
Kong, Xiangang
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-04
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Newcastle disease (ND), caused by Newcastle disease virus (NDV), is highly contagious and represents a major threat to the poultry industry.
The thermostable vaccines are not insensitive to heat and ease of storage and transportation, but the mechanism of NDV thermostability remains unknown.
The phosphoprotein (P), fusion protein (F), hemagglutinin-neuraminidase protein (HN), and large polymerase protein (L) are associated with NDV virulence.
The association between F, HN, or L and viral thermostability has been, respectively, studied in different reports.
However, the effects of P on NDV thermostability have not been demonstrated.
Here, we utilized an existing reverse genetics system in our laboratory, to generate chimeric viruses by exchanging the P protein between the thermostable NDV4-C strain and the thermolabile LaSota strain.
Chimeric viruses were found to possess similar growth properties, passage stability, and virulence, as compared to those of these parental strains.
Interestingly, the thermostability of the chimera with P derived from the thermolabile LaSota strain was reduced compared to that of the parental virus, and P of the thermostable NDV4-C strain enhanced chimeric virus thermostability.
Our data demonstrate that P is an important factor for the thermostability of NDV and provides information regarding the molecular mechanism of NDV thermostability; moreover, these results suggest a theoretical basis for using the NDV4-C strain as a thermostable vaccine.
American Psychological Association (APA)
Zhao, Yang& Liu, Huairan& Cong, Feng& Wu, Wei& Zhao, Ran& Kong, Xiangang. 2018. Phosphoprotein Contributes to the Thermostability of Newcastle Disease Virus. BioMed Research International،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1129363
Modern Language Association (MLA)
Zhao, Yang…[et al.]. Phosphoprotein Contributes to the Thermostability of Newcastle Disease Virus. BioMed Research International No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1129363
American Medical Association (AMA)
Zhao, Yang& Liu, Huairan& Cong, Feng& Wu, Wei& Zhao, Ran& Kong, Xiangang. Phosphoprotein Contributes to the Thermostability of Newcastle Disease Virus. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1129363
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129363