Impact of Hypothermia and Oxygen Deprivation on the Cytoskeleton in Organ Preservation Models
Joint Authors
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Ischemia reperfusion (IR) lesions are an unavoidable consequence of organ transplantation.
Researching new therapeutics against these lesions requires the definition of early mechanisms.
The cytoskeleton is composed of 3 types of filaments: microfilaments, intermediate filaments, and microtubules.
We aimed to characterize the influence of preservation on their phenotype.
In an in vitro model using primary human endothelial cells reproducing the conditions of organ preservation, two aspects were explored: (a) the impact of IR and cold ischemia time on each filament type, evaluating the roles of temperature, solution, and oxygen; and (b) the potential of cytoskeleton-mediated therapy to alleviate cell death.
Results showed that intermediary filaments were unaffected, while microfilaments showed radical changes with disappearance of the structure replaced by a disorganized array of nodules; moreover, microtubules almost completely disappeared with time.
Furthermore, temperature, and not oxygen deprivation or the solution, was the determining factor of the cytoskeleton’s loss of integrity during preservation.
Finally, pharmaceutical intervention could indeed preserve fiber structure but did not alter survival.
Our work shows that improvement of preservation must include a more adapted temperature before considering oxygen, as it could profoundly improve cytoskeleton organization and thus cell fate.
This highlights the importance of this structure for the development of new therapeutics and the definition of graft quality biomarkers.
American Psychological Association (APA)
Thuillier, Raphael& Hauet, T.. 2018. Impact of Hypothermia and Oxygen Deprivation on the Cytoskeleton in Organ Preservation Models. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1129375
Modern Language Association (MLA)
Thuillier, Raphael& Hauet, T.. Impact of Hypothermia and Oxygen Deprivation on the Cytoskeleton in Organ Preservation Models. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1129375
American Medical Association (AMA)
Thuillier, Raphael& Hauet, T.. Impact of Hypothermia and Oxygen Deprivation on the Cytoskeleton in Organ Preservation Models. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1129375
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129375