Biomimetic Implant Surface Functionalization with Liquid L-PRF Products: In Vitro Study
Joint Authors
De Biase, Alberto
Formisano, Giuseppe
Maritato, Manuela
Bozzuto, Giuseppina
Molinari, Agnese
Lollobrigida, Marco
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
Platelet-rich fibrin (PRF) clots and membranes are autologous blood concentrates widely used in oral surgical procedures; less is known, however, about the liquid formulations of such products.
The aim of this in vitro study is to assess the behavior of different implant surfaces when in contact with two liquid leucocyte- and platelet-rich fibrin (L-PRF) products.
Methods.
Six commercial pure titanium discs, of 9.5 mm diameter and 1.5 mm thickness, were used.
Three of these samples had a micro/nano-rough surface; three were machined.
Three different protocols were tested.
Protocols involved the immersion of the samples in (1) a platelets, lymphocytes, and fibrinogen liquid concentrate (PLyF) for 10 minutes, (2) an exudate obtained from L-PRF clots rich in fibronectin and vitronectin for 5 minutes, and (3) the fibronectin/vitronectin exudate for 2 minutes followed by immersion in the PLyF concentrate for further 8 minutes.
After these treatments, the samples were fixed and observed using a scanning electron microscope (SEM).
Results.
Under microscopic observation, (1) the samples treated with the PLyF concentrate revealed a dense fibrin network in direct contact with the implant surface and a significant number of formed elements of blood; (2) in the samples treated with the fibronectin/vitronectin exudates, only a small number of white and red blood cells were detectable; and (3) in samples exposed to the combined treatment, there was an apparent increase in the thickness of the fibrin layer.
When compared to the machined surface, the micro/nano-rough samples showed an overall increased retention of fibrin, leading to a thicker coating.
Conclusions.
Liquid L-PRF products promote the formation of a dense fibrin clot on micro/nano-rough implant surfaces in vitro.
The adjunctive treatment of surfaces with the fibronectin/vitronectin exudate could provide support to contact of the fibrin with the surface, though it is not essential for the clot formation.
Further studies are necessary to better elucidate the properties and benefits of liquid L-PRF products.
American Psychological Association (APA)
Lollobrigida, Marco& Maritato, Manuela& Bozzuto, Giuseppina& Formisano, Giuseppe& Molinari, Agnese& De Biase, Alberto. 2018. Biomimetic Implant Surface Functionalization with Liquid L-PRF Products: In Vitro Study. BioMed Research International،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1129441
Modern Language Association (MLA)
Lollobrigida, Marco…[et al.]. Biomimetic Implant Surface Functionalization with Liquid L-PRF Products: In Vitro Study. BioMed Research International No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1129441
American Medical Association (AMA)
Lollobrigida, Marco& Maritato, Manuela& Bozzuto, Giuseppina& Formisano, Giuseppe& Molinari, Agnese& De Biase, Alberto. Biomimetic Implant Surface Functionalization with Liquid L-PRF Products: In Vitro Study. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1129441
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129441