Identification of Differentially Expressed Genes in Porcine Ovaries at Proestrus and Estrus Stages Using RNA-Seq Technique
Joint Authors
Yang, Songbai
Zhou, Xiaolong
Pei, Yue
Wang, Han
He, Ke
Zhao, Ayong
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Estrus is an important factor for the fecundity of sows, and it is involved in ovulation and hormone secretion in ovaries.
To better understand the molecular mechanisms of porcine estrus, the expression patterns of ovarian mRNA at proestrus and estrus stages were analyzed using RNA sequencing technology.
A total of 2,167 differentially expressed genes (DEGs) were identified (P≤0.05, log2 Ratio≥1), of which 784 were upregulated and 1,383 were downregulated in the estrus compared with the proestrus group.
Gene Ontology (GO) enrichment indicated that these DEGs were mainly involved in the cellular process, single-organism process, cell and cell part, and binding and metabolic process.
In addition, a pathway analysis showed that these DEGs were significantly enriched in 33 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including cell adhesion molecules, ECM-receptor interaction, and cytokine-cytokine receptor interaction.
Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) confirmed the differential expression of 10 selected DEGs.
Many of the novel candidate genes identified in this study will be valuable for understanding the molecular mechanisms of the sow estrous cycle.
American Psychological Association (APA)
Yang, Songbai& Zhou, Xiaolong& Pei, Yue& Wang, Han& He, Ke& Zhao, Ayong. 2018. Identification of Differentially Expressed Genes in Porcine Ovaries at Proestrus and Estrus Stages Using RNA-Seq Technique. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1129524
Modern Language Association (MLA)
Yang, Songbai…[et al.]. Identification of Differentially Expressed Genes in Porcine Ovaries at Proestrus and Estrus Stages Using RNA-Seq Technique. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1129524
American Medical Association (AMA)
Yang, Songbai& Zhou, Xiaolong& Pei, Yue& Wang, Han& He, Ke& Zhao, Ayong. Identification of Differentially Expressed Genes in Porcine Ovaries at Proestrus and Estrus Stages Using RNA-Seq Technique. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1129524
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129524