Identification of Six Potentially Long Noncoding RNAs as Biomarkers Involved Competitive Endogenous RNA in Clear Cell Renal Cell Carcinoma
Joint Authors
Yang, Kang
Lu, Xiao-fan
Luo, Peng-cheng
Zhang, Jie
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-11
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background.
Clear cell renal cell carcinoma (ccRCC), the most common subtype of renal cell carcinoma (RCC), usually is representative of metastatic heterogeneous neoplasm that links with poor prognosis, but the pathogenesis of ccRCC remains unclear.
Currently, numerous evidences prove that long noncoding RNAs (lncRNAs) are considered as competing endogenous RNA (ceRNA) to participate in cellular processes of tumors.
Therefore, to investigate the underlying mechanisms of ccRCC, the expression profiles of lncRNAs, miRNAs, and mRNAs were downloaded from the Cancer Genome Atlas (TCGA) database.
A total of 1526 differentially expressed lncRNAs (DElncRNAs), 54 DEmiRNAs, and 2352 DEmRNAs were identified.
To determine the connection of them, all DElncRNAs were input to the miRcode database.
The results indicated that 85 DElncRNAs could connect with 9 DEmiRNAs in relation to our study.
Then, databases of TargetScan and miRDB were used to search for targeted genes with reference to DEmiRNAs.
The results showed that 203 out of 2352 targeted genes were identified in our TCGA set.
Subsequently, ceRNA network was constructed according to Cytoscape and the targeted genes were functionally analyzed to elucidate the mechanisms of DEmRNAs.
The results of survival analysis and regression analysis indicated that 6 DElncRNAs named COL18A1-AS1, WT1-AS, LINC00443, TCL6, AL356356.1, and SLC25A5-AS1 were significantly correlative with the clinical traits of ccRCC patients and could be served as predictors for ccRCC.
Finally, these findings were validated by quantitative RT-PCR (qRT-PCR).
Based on these discoveries, we believe that this identified ceRNA network will provide a novel perspective to elucidate ccRCC pathogenesis.
American Psychological Association (APA)
Yang, Kang& Lu, Xiao-fan& Luo, Peng-cheng& Zhang, Jie. 2018. Identification of Six Potentially Long Noncoding RNAs as Biomarkers Involved Competitive Endogenous RNA in Clear Cell Renal Cell Carcinoma. BioMed Research International،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1129658
Modern Language Association (MLA)
Yang, Kang…[et al.]. Identification of Six Potentially Long Noncoding RNAs as Biomarkers Involved Competitive Endogenous RNA in Clear Cell Renal Cell Carcinoma. BioMed Research International No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1129658
American Medical Association (AMA)
Yang, Kang& Lu, Xiao-fan& Luo, Peng-cheng& Zhang, Jie. Identification of Six Potentially Long Noncoding RNAs as Biomarkers Involved Competitive Endogenous RNA in Clear Cell Renal Cell Carcinoma. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1129658
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129658