Profiling of StemProgenitor Cell Regulatory Genes of the Synovial Joint by Genome-Wide RNA-Seq Analysis
Joint Authors
Zhou, Xuedong
Zhou, Yue
Chen, Mo
Ricupero, Christopher L.
He, Ling
Wu, Jiaqian
Chen, Kenian
Friedman, Richard A.
Guarnieri, Paolo
Wang, Zuolin
Mao, Jeremy J.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Synovial joints suffer from arthritis and trauma that may be severely debilitative.
Despite robust investigations in the roles of individual genes in synovial joint development and arthritis, little is known about global profiles of genes that regulate stem/progenitor cells of a synovial joint.
The temporomandibular joint is a poorly understood synovial arthrosis with few clinical treatment options.
Here, we isolated the articular and mature zones of the mandibular condyle by laser capture microdissection, performed genome-wide profiling, and analyzed molecular signaling pathways relevant to stem/progenitor cell functions.
A total of 804 genes were differentially expressed between the articular and mature zones.
Pathway analyses revealed 29 enriched signaling pathways, including the PI3K-Akt, Wnt, and Toll-like receptor signaling pathways that may regulate stem/progenitor cell homeostasis and differentiation into the chondrocyte lineage.
Upstream regulator analyses further predicted potential upstream key regulators such as Xbp1, Nupr1, and Hif1a, and associated underlying mechanism networks were described.
Among the multiple candidates of growth and transcriptional factors that may regulate stem/progenitor cells, we immunolocalized Sox9, Ihh, Frzb, Dkk1, Lgr5, and TGFβ3 in the articular and mature zones.
These findings provide a comprehensive genetic mapping of growth and transcriptional genes in the articular and mature zones of a synovial joint condyle.
Differentially expressed genes may play crucial roles in the regulation of stem/progenitor cells in development, homeostasis, and tissue regeneration.
American Psychological Association (APA)
Zhou, Yue& Chen, Mo& Ricupero, Christopher L.& He, Ling& Wu, Jiaqian& Chen, Kenian…[et al.]. 2018. Profiling of StemProgenitor Cell Regulatory Genes of the Synovial Joint by Genome-Wide RNA-Seq Analysis. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1129671
Modern Language Association (MLA)
Zhou, Yue…[et al.]. Profiling of StemProgenitor Cell Regulatory Genes of the Synovial Joint by Genome-Wide RNA-Seq Analysis. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1129671
American Medical Association (AMA)
Zhou, Yue& Chen, Mo& Ricupero, Christopher L.& He, Ling& Wu, Jiaqian& Chen, Kenian…[et al.]. Profiling of StemProgenitor Cell Regulatory Genes of the Synovial Joint by Genome-Wide RNA-Seq Analysis. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1129671
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129671