Diethylnitrosamine Increases Proliferation in Early Stages of Hepatic Carcinogenesis in Insulin-Treated Type 1 Diabetic Mice
Joint Authors
Carnovale, Cristina E.
Arboatti, A. S.
Lambertucci, F.
Sedlmeier, M. G.
Pisani, G.
Monti, J.
Álvarez, M. de L.
Francés, D. E. A.
Ronco, M. T.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Diethylnitrosamine (DEN) induces hepatocarcinogenesis, increasing mitotic hepatocytes and leading to chronic inflammation.
In addition, type 1 diabetes mellitus (T1DM) is also characterized by a proinflammatory state and by requiring insulin exogenous treatment.
Given the association of diabetes, insulin treatment, and cell proliferation, our specific goal was to determine whether the liver in the diabetic state presents a greater response to DEN-induced cell cycle alteration, which is essential for the malignant transformation.
Male C57BL/6 mice (four-week-old) were divided into 4 groups: C, C + DEN, T1DM, and T1DM + DEN.
Mice were euthanized ten weeks after DEN injection.
DEN per se produced an increase in liver lipid peroxidation levels.
Besides, in T1DM + DEN, we found a greater increase in the proliferation index, in comparison with C + DEN.
These results are in agreement with the increased expression observed in cell cycle progression markers: cyclin D1 and E1.
In addition, a proapoptotic factor, such as activated caspase-3, evidenced a decrease in T1DM + DEN, while the Vascular Endothelial Growth Factor (VEGF) and the protooncogene p53 showed a higher increase with respect to C + DEN.
Overall, the results allow us to highlight a major DEN response in T1DM, which may explain in part the greater predisposition to the development of hepatocarcinoma (HCC) during the diabetic state.
American Psychological Association (APA)
Arboatti, A. S.& Lambertucci, F.& Sedlmeier, M. G.& Pisani, G.& Monti, J.& Álvarez, M. de L.…[et al.]. 2018. Diethylnitrosamine Increases Proliferation in Early Stages of Hepatic Carcinogenesis in Insulin-Treated Type 1 Diabetic Mice. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1129782
Modern Language Association (MLA)
Arboatti, A. S.…[et al.]. Diethylnitrosamine Increases Proliferation in Early Stages of Hepatic Carcinogenesis in Insulin-Treated Type 1 Diabetic Mice. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1129782
American Medical Association (AMA)
Arboatti, A. S.& Lambertucci, F.& Sedlmeier, M. G.& Pisani, G.& Monti, J.& Álvarez, M. de L.…[et al.]. Diethylnitrosamine Increases Proliferation in Early Stages of Hepatic Carcinogenesis in Insulin-Treated Type 1 Diabetic Mice. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1129782
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129782