Helicobacter pylori Mutations Conferring Resistance to Fluoroquinolones and Clarithromycin among Dyspeptic Patients Attending a Tertiary Hospital, Tanzania
Joint Authors
Mshana, Stephen E.
Jaka, Hyasinta
Rüttgerodt, Nele
Bohne, Wolfgang
Mueller, Andreas
Kasang, Christa
Groß, Uwe
Source
Canadian Journal of Gastroenterology and Hepatology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objectives.
Helicobacter pylori (H.
pylori) isolates resistant to clarithromycin and quinolones are increasing worldwide.
Data regarding the magnitude of H.
pylori resistance are limited in developing countries.
Here, we report the prevalence of mutations conferring resistance to clarithromycin and fluoroquinolones among dyspeptic patients attending a tertiary hospital, Tanzania.
Methods.
Between August 2014 and August 2016, patients undergoing upper gastrointestinal endoscopy at the Bugando Medical Centre were enrolled.
Biopsies were taken for polymerase chain reaction (PCR) and sequencing to detect mutations conferring resistance to clarithromycin and fluoroquinolones.
Results.
A total of 208 nonrepetitive biopsies were examined of which 188 (90.4%) tested positive for H.
pylori specific 23S rRNA PCR.
Clarithromycin resistance mutations were detected in 54/188 (28.7%) of patients tested.
The most frequently detected mutation was A2143G (30) followed by A2142G (20).
Out of 131 nonrepetitive biopsies tested for fluoroquinolones resistance mutations, 77/131 (58.8%) were positive, with N87I (20) mutation being the most frequently detected mutation followed by A92T mutation which was detected in 16 samples.
Conclusion.
A significant proportion of dyspeptic patients attending tertiary hospital in Tanzania are infected with H.
pylori strains harbouring clarithromycin or fluoroquinolones resistance mutations.
Detection of more than 50% of strains with fluoroquinolones resistance mutations makes the H.
pylori second line treatment questionable in our setting.
There is a need of surveillance of H.
pylori resistance patterns in Tanzania to provide data that can guide empirical treatment to reduce associated morbidity of H.
pylori infections.
The correlation between A92T fluoroquinolone mutation and phenotypic resistance requires further investigations.
American Psychological Association (APA)
Jaka, Hyasinta& Rüttgerodt, Nele& Bohne, Wolfgang& Mueller, Andreas& Groß, Uwe& Kasang, Christa…[et al.]. 2019. Helicobacter pylori Mutations Conferring Resistance to Fluoroquinolones and Clarithromycin among Dyspeptic Patients Attending a Tertiary Hospital, Tanzania. Canadian Journal of Gastroenterology and Hepatology،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1129917
Modern Language Association (MLA)
Jaka, Hyasinta…[et al.]. Helicobacter pylori Mutations Conferring Resistance to Fluoroquinolones and Clarithromycin among Dyspeptic Patients Attending a Tertiary Hospital, Tanzania. Canadian Journal of Gastroenterology and Hepatology No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1129917
American Medical Association (AMA)
Jaka, Hyasinta& Rüttgerodt, Nele& Bohne, Wolfgang& Mueller, Andreas& Groß, Uwe& Kasang, Christa…[et al.]. Helicobacter pylori Mutations Conferring Resistance to Fluoroquinolones and Clarithromycin among Dyspeptic Patients Attending a Tertiary Hospital, Tanzania. Canadian Journal of Gastroenterology and Hepatology. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1129917
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129917