Neuroprotection by Paeoniflorin against Nuclear Factor Kappa B-Induced Neuroinflammation on Spinal Cord Injury
Joint Authors
Geng, Wujun
Wang, Bin
Dai, Wangying
Shi, Lijun
Teng, Honglin
Li, Xigong
Wang, Jing
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Acute spinal cord injury (SCI) is one of the most common and devastating causes of sensory or motor dysfunction.
Nuclear factor-kappa B(NF-κB)-mediated neuroinflammatory responses, in addition to nitric oxide (NO), are key regulatory pathways in SCI.
Paeoniflorin (PF), a major active component extracted from Paeonia roots, has been suggested to exert neuroprotective effects in the central nervous system.
However, whether PF could improve the motor function after SCI in vivo is still unclear.
Method.
Immunohistochemical analysis, western blot, real-time quantitative PCR, immunofluorescence staining, and histopathological and behavioral evaluation were used to explore the effects of paeoniflorin after SCI for 14 days.
Results.
In this study, PF treatment significantly inhibited NF-κB activation and downregulated the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2(COX-2), and Nogo-A.
Comparing behavioral and histological changes in SCI and PF treatment groups, we found that PF treatment improved motor function recovery, attenuated the histopathological damage, and increased neuronal survival in the SCI model.
PF treatment also reduced expression levels of Bax and c-caspase-3 and increased the expression level of Bcl-2 and cell viabilities.
Upregulation of TNF-α, IL-6, and IL-1β after injury was also prevented by PF.
Conclusion.
These results suggest that the neuroprotective effects of PF are related to the inhibition of the NF-κB signaling pathway.
And PF may be a therapeutic strategy in spinal cord injury.
American Psychological Association (APA)
Wang, Bin& Dai, Wangying& Shi, Lijun& Teng, Honglin& Li, Xigong& Wang, Jing…[et al.]. 2018. Neuroprotection by Paeoniflorin against Nuclear Factor Kappa B-Induced Neuroinflammation on Spinal Cord Injury. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1130121
Modern Language Association (MLA)
Wang, Bin…[et al.]. Neuroprotection by Paeoniflorin against Nuclear Factor Kappa B-Induced Neuroinflammation on Spinal Cord Injury. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1130121
American Medical Association (AMA)
Wang, Bin& Dai, Wangying& Shi, Lijun& Teng, Honglin& Li, Xigong& Wang, Jing…[et al.]. Neuroprotection by Paeoniflorin against Nuclear Factor Kappa B-Induced Neuroinflammation on Spinal Cord Injury. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1130121
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130121