An Evaluation of the Antibacterial Properties of Tormentic Acid Congener and Extracts From Callistemon viminalis on Selected ESKAPE Pathogens and Effects on Biofilm Formation
Joint Authors
Chipenzi, Tafadzwa
Baloyi, Genuine
Mudondo, Tatenda
Sithole, Simbarashe
Fru Chi, Godloves
Mukanganyama, Stanley
Source
Advances in Pharmacological and Pharmaceutical Sciences
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-09
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
ESKAPE pathogens, namely, Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species, are responsible for a majority of all healthcare-acquired infections (HAI).
The bacteria cause nosocomial infections in immunocompromised patients.
Extracts from Callistemon viminalis have been shown to have antibacterial, antifungal, and anti-inflammatory activities.
Tormentic acid congener, a pentacyclic triterpene saponin, was isolated from C.
viminalis leaves.
This study aimed to investigate the antibacterial effects of tormentic acid congener and leaf extracts on biofilm formation by A.
baumannii, S.
aureus, S.
pyogenes, and P.
aeruginosa.
The antibacterial effects were determined by the microbroth dilution method, and ciprofloxacin was used as the standard antibacterial drug.
Biofilm formation and detachment assays were performed using crystal violet staining.
Production of extracellular polymeric DNA and polysaccharides from biofilms was also determined.
Tormentic acid congener showed time-dependent antibacterial activity against P.
aeruginosa with a MIC of 100 µg/ml and caused significant protein leakage.
Antibacterial activity was found when tormentic acid congener was tested against both S.
aureus and P.
aeruginosa.
The MICs were found to be 25 µg/ml and 12.5 µg/ml for P.
aeruginosa and S.
aureus cells, respectively.
S.
pyogenes was found to be susceptible to tormentic acid congener and the hydroethanolic extract with an MIC of 100 µg/ml and 25 µg/ml, respectively.
A.
baumannii was found not to be susceptible to the compound or the extracts.
The compound and the extracts caused a significant decrease in the biofilm extracellular polysaccharide content of S.
pyogenes.
The extracts and tormentic acid congener caused detachment of biofilms and decreased the release of extracellular DNA and capsular polysaccharides from biofilms of P.
aeruginosa and S.
aureus.
Tormentic acid congener and extracts, thus, have significant antibacterial and antibiofilm activities on these selected ESKAPE bacteria and can act as source lead compounds for the development of antibacterial triterpenoids.
American Psychological Association (APA)
Chipenzi, Tafadzwa& Baloyi, Genuine& Mudondo, Tatenda& Sithole, Simbarashe& Fru Chi, Godloves& Mukanganyama, Stanley. 2020. An Evaluation of the Antibacterial Properties of Tormentic Acid Congener and Extracts From Callistemon viminalis on Selected ESKAPE Pathogens and Effects on Biofilm Formation. Advances in Pharmacological and Pharmaceutical Sciences،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1130124
Modern Language Association (MLA)
Chipenzi, Tafadzwa…[et al.]. An Evaluation of the Antibacterial Properties of Tormentic Acid Congener and Extracts From Callistemon viminalis on Selected ESKAPE Pathogens and Effects on Biofilm Formation. Advances in Pharmacological and Pharmaceutical Sciences No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1130124
American Medical Association (AMA)
Chipenzi, Tafadzwa& Baloyi, Genuine& Mudondo, Tatenda& Sithole, Simbarashe& Fru Chi, Godloves& Mukanganyama, Stanley. An Evaluation of the Antibacterial Properties of Tormentic Acid Congener and Extracts From Callistemon viminalis on Selected ESKAPE Pathogens and Effects on Biofilm Formation. Advances in Pharmacological and Pharmaceutical Sciences. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1130124
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130124