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Chimeric Japanese Encephalitis Virus SA14SA14-14-2 Was Virulence Attenuated and Protected the Challenge of Wild-Type Strain SA14
Joint Authors
Huang, Rong
Leng, Shengling
Feng, Yalan
Tang, Liping
Yuan, Lei
Yang, Jian
Source
Canadian Journal of Infectious Diseases and Medical Microbiology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The attenuated Japanese encephalitis virus (JEV) live vaccine SA14-14-2 prepared from wild-type (WT) strain SA14 was licensed to prevent Japanese encephalitis (JE) in 1989 in China.
Many studies showed that the premembrane (prM) and envelope (E) protein were the crucial determinant of virulence and immunogenicity of JEV.
So we are interested in whether the substitution of prM/E of JEV WT SA14 with those of vaccine strain SA14-14-2 could decrease neurovirulence and prevent the challenge of JEV WT SA14.
Molecular clone technique was used to replace the prM/E gene of JEV WT strain SA14 with those of vaccine strain SA14-14-2 to construct the infectious clone of chimeric virus (designated JEV SA14/SA14-14-2), the chimeric virus recovered from BHK21 cells upon electrotransfection of RNA into BHK21 cells.
The results showed that the recovered chimeric virus was highly attenuated in mice, and a single immunization elicited strong protective immunity in a dose-dependent manner.
This study increases our understanding of the molecular mechanisms of neurovirulence attenuation and immunogenicity of JEV.
American Psychological Association (APA)
Huang, Rong& Leng, Shengling& Feng, Yalan& Tang, Liping& Yuan, Lei& Yang, Jian. 2019. Chimeric Japanese Encephalitis Virus SA14SA14-14-2 Was Virulence Attenuated and Protected the Challenge of Wild-Type Strain SA14. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1130126
Modern Language Association (MLA)
Huang, Rong…[et al.]. Chimeric Japanese Encephalitis Virus SA14SA14-14-2 Was Virulence Attenuated and Protected the Challenge of Wild-Type Strain SA14. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1130126
American Medical Association (AMA)
Huang, Rong& Leng, Shengling& Feng, Yalan& Tang, Liping& Yuan, Lei& Yang, Jian. Chimeric Japanese Encephalitis Virus SA14SA14-14-2 Was Virulence Attenuated and Protected the Challenge of Wild-Type Strain SA14. Canadian Journal of Infectious Diseases and Medical Microbiology. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1130126
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130126