MiR-92a Inhibits the Progress of Osteosarcoma Cells and Increases the Cisplatin Sensitivity by Targeting Notch1
Joint Authors
Liu, Quanxiang
Song, Yang
Duan, Xianliang
Chang, Yuan
Guo, Jianping
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
MicroRNAs (miRs) have been implicated in the development and progression of osteosarcoma.
Here, we aimed to illustrate the important role of miR-92a on the regulation of OS development which may help to establish a novel strategy for OS diagnosis and treatment.
Materials and Methods.
Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
Cell cycle and apoptosis were assessed by flow cytometry with PI and PI/Annexin-V stain, respectively.
The expression of proteins was examined by western blot.
qPCR was used to detect the expression of RNA.
Cell migration was assayed with transwell assay.
Results.
MiR-92a inhibited the proliferation and the migration of OS in vitro and reduced the volume of the tumour in vivo.
Further, miR-92a enhanced cisplatin sensitivity of OS.
MiR-92a directly targeted Notch1.
Conclusion.
Together, our results indicate that miR-92a inhibited cell growth, migration, and enhanced cisplatin sensitivity of OS cell by targeting Notch1.
American Psychological Association (APA)
Liu, Quanxiang& Song, Yang& Duan, Xianliang& Chang, Yuan& Guo, Jianping. 2018. MiR-92a Inhibits the Progress of Osteosarcoma Cells and Increases the Cisplatin Sensitivity by Targeting Notch1. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1130132
Modern Language Association (MLA)
Liu, Quanxiang…[et al.]. MiR-92a Inhibits the Progress of Osteosarcoma Cells and Increases the Cisplatin Sensitivity by Targeting Notch1. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1130132
American Medical Association (AMA)
Liu, Quanxiang& Song, Yang& Duan, Xianliang& Chang, Yuan& Guo, Jianping. MiR-92a Inhibits the Progress of Osteosarcoma Cells and Increases the Cisplatin Sensitivity by Targeting Notch1. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1130132
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130132