An Efficient T1 Contrast Agent for Labeling and Tracking Human Embryonic Stem Cells on MRI
Joint Authors
Haedicke, Inga E.
Loai, Sadi
Margaret Cheng, Hai-Ling
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Noninvasive cell tracking in vivo has the potential to advance stem cell-based therapies into the clinic.
Magnetic resonance imaging (MRI) provides an excellent image-guidance platform; however, existing MR cell labeling agents are fraught with limited specificity.
To address this unmet need, we developed a highly efficient manganese porphyrin contrast agent, MnEtP, using a two-step synthesis.
In vitro MRI at 3 Tesla on human embryonic stem cells (hESCs) demonstrated high labeling efficiency at a very low dose of 10 µM MnEtP, resulting in a four-fold lower T1 relaxation time.
This extraordinarily low dose is ideal for labeling large cell numbers required for large animals and humans.
Cell viability and differentiation capacity were unaffected.
Cellular manganese quantification corroborated MRI findings, and the agent localized primarily on the cell membrane.
In vivo MRI of transplanted hESCs in a rat demonstrated excellent sensitivity and specificity of MnEtP for noninvasive stem cell tracking.
American Psychological Association (APA)
Haedicke, Inga E.& Loai, Sadi& Margaret Cheng, Hai-Ling. 2019. An Efficient T1 Contrast Agent for Labeling and Tracking Human Embryonic Stem Cells on MRI. Contrast Media & Molecular Imaging،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1130221
Modern Language Association (MLA)
Haedicke, Inga E.…[et al.]. An Efficient T1 Contrast Agent for Labeling and Tracking Human Embryonic Stem Cells on MRI. Contrast Media & Molecular Imaging No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1130221
American Medical Association (AMA)
Haedicke, Inga E.& Loai, Sadi& Margaret Cheng, Hai-Ling. An Efficient T1 Contrast Agent for Labeling and Tracking Human Embryonic Stem Cells on MRI. Contrast Media & Molecular Imaging. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1130221
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130221