Neuroprotective Effect of DAHP via Antiapoptosis in Cerebral Ischemia
Joint Authors
Yang, Yang
Li, Weiyun
Qin, Yanhua
Hu, Weiming
Fang, Marong
Hu, Zhiying
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-19
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Aberrant production of nitric oxide following inducible nitric oxide synthase (iNOS) expression has been implicated in cell death and contributes to ischemic brain injury.
Tetrahydrobiopterin (BH4) is an essential cofactor of NOS activity.
Herein, we evaluated antiapoptotic and anti-inflammatory effects of diamino-6-hydroxypyrimidine (DAHP), a guanosine 5′-triphosphate cyclohydrolase 1 (GTPCH1) inhibitor on focal cerebral ischemia-reperfusion injury by middle cerebral artery occlusion and reperfusion (MCAO) and investigated the underlying mechanism.
Sprague-Dawley rats were divided into five groups.
Experimental groups were subjected to 1.5 h transient MCAO.
T2-weighted imaging was performed to evaluate brain edema lesions in the stroke rats.
Infarct volume was estimated by 2,3,5-triphenyltetrazolium chloride (TTC) staining after 24 h reperfusion.
Western blotting and immunohistochemistry were performed to detect iNOS, caspase-3, Bcl-2, COX-2, and TNF-α protein expressions.
Apoptosis was determined by TUNEL staining.
T2 hyperintensity changes were observed in primary ischemic region.
DAHP pretreatment significantly suppressed iNOS overexpression, caspase-3, and TNF-α.
There was also attenuation of neuronal apoptosis with decrement in proteins Bcl-2 and COX-2 expressions.
On the basis of our results, we hypothesize DAHP to have a neuroprotective function against focal cerebral ischemia and might attenuate brain injury by decreasing reactive oxygen species (ROS) production, subsequently inhibiting apoptosis.
American Psychological Association (APA)
Qin, Yanhua& Hu, Weiming& Yang, Yang& Hu, Zhiying& Li, Weiyun& Fang, Marong. 2018. Neuroprotective Effect of DAHP via Antiapoptosis in Cerebral Ischemia. Behavioural Neurology،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1130238
Modern Language Association (MLA)
Qin, Yanhua…[et al.]. Neuroprotective Effect of DAHP via Antiapoptosis in Cerebral Ischemia. Behavioural Neurology No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1130238
American Medical Association (AMA)
Qin, Yanhua& Hu, Weiming& Yang, Yang& Hu, Zhiying& Li, Weiyun& Fang, Marong. Neuroprotective Effect of DAHP via Antiapoptosis in Cerebral Ischemia. Behavioural Neurology. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1130238
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130238