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Detection of Active Caspase-3 in Mouse Models of Stroke and Alzheimer’s Disease with a Novel Dual Positron Emission TomographyFluorescent Tracer [68Ga]Ga-TC3-OGDOTA
Joint Authors
Ostapchenko, Valeriy G.
Snir, Jonatan
Suchy, Mojmir
Fan, Jue
Cobb, M. Rebecca
Chronik, Blaine A.
Kovacs, Michael
Prado, Vania F.
Hudson, Robert H. E.
Pasternak, Stephen H.
Bartha, Robert
Prado, M.
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-14
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Apoptosis is a feature of stroke and Alzheimer’s disease (AD), yet there is no accepted method to detect or follow apoptosis in the brain in vivo.
We developed a bifunctional tracer [68Ga]Ga-TC3-OGDOTA containing a cell-penetrating peptide separated from fluorescent Oregon Green and 68Ga-bound labels by the caspase-3 recognition peptide DEVD.
We hypothesized that this design would allow [68Ga]Ga-TC3-OGDOTA to accumulate in apoptotic cells.
In vitro, Ga-TC3-OGDOTA labeled apoptotic neurons following exposure to camptothecin, oxygen-glucose deprivation, and β-amyloid oligomers.
In vivo, PET showed accumulation of [68Ga]Ga-TC3-OGDOTA in the brain of mouse models of stroke or AD.
Optical clearing revealed colocalization of [68Ga]Ga-TC3-OGDOTA and cleaved caspase-3 in brain cells.
In stroke, [68Ga]Ga-TC3-OGDOTA accumulated in neurons in the penumbra area, whereas in AD mice [68Ga]Ga-TC3-OGDOTA was found in single cells in the forebrain and diffusely around amyloid plaques.
In summary, this bifunctional tracer is selectively associated with apoptotic cells in vitro and in vivo in brain disease models and represents a novel tool for apoptosis detection that can be used in neurodegenerative diseases.
American Psychological Association (APA)
Ostapchenko, Valeriy G.& Snir, Jonatan& Suchy, Mojmir& Fan, Jue& Cobb, M. Rebecca& Chronik, Blaine A.…[et al.]. 2019. Detection of Active Caspase-3 in Mouse Models of Stroke and Alzheimer’s Disease with a Novel Dual Positron Emission TomographyFluorescent Tracer [68Ga]Ga-TC3-OGDOTA. Contrast Media & Molecular Imaging،Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1130329
Modern Language Association (MLA)
Ostapchenko, Valeriy G.…[et al.]. Detection of Active Caspase-3 in Mouse Models of Stroke and Alzheimer’s Disease with a Novel Dual Positron Emission TomographyFluorescent Tracer [68Ga]Ga-TC3-OGDOTA. Contrast Media & Molecular Imaging No. 2019 (2019), pp.1-17.
https://search.emarefa.net/detail/BIM-1130329
American Medical Association (AMA)
Ostapchenko, Valeriy G.& Snir, Jonatan& Suchy, Mojmir& Fan, Jue& Cobb, M. Rebecca& Chronik, Blaine A.…[et al.]. Detection of Active Caspase-3 in Mouse Models of Stroke and Alzheimer’s Disease with a Novel Dual Positron Emission TomographyFluorescent Tracer [68Ga]Ga-TC3-OGDOTA. Contrast Media & Molecular Imaging. 2019. Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1130329
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130329