Improved Therapeutic Effect of Puerarin-Encapsulated PEG-PLGA Nanoparticle on an In Vitro Cerebral Infarction Model
Joint Authors
Li, Lei
Li, Yan
Miao, Cheng
Liu, Rui
Source
Advances in Polymer Technology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-28
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study was to explore the therapeutic effect and mechanism of puerarin (PUE) combined with PEGylated nanoparticles on a rat cerebral infarction cell model.
In this context, PEG-PLGA/PUE nanoparticles were prepared by the thin-film hydration method, and the toxicity of PEG-PLGA/PUE nanoparticles to brain capillary endothelial cell (BCEC) was detected by MTT.
The BCEC/TF cell model was obtained by induction of BCEC cells with TNF-α.
The BCEC/TF cell model was identified by immunofluorescence; the protein expression was detected by western blotting; the expression level of miR-424 in cells was measured by RT-qPCR; the targeting relationship between miR-424 and PDCD4 was confirmed by dual-luciferase reporter assay.
We found that PEG-PLGA/PUE nanoparticles prepared by the thin-film hydration method had uniform particle size, regular shape, and good stability and were not toxic to cells.
The vWF was widely expressed in the cytoplasm in BCECs.
The BCEC/TF cell model was obtained after TNF-α treatment, and tissue factor (TF) was widely expressed on the cell membrane of BCEC/TF cells.
Furthermore, it was observed that the PEG-PLGA/PUE nanoparticles showed better therapeutic effect on the BCEC/TF cell model than PUE.
PEG-PLGA/PUE nanoparticles and PUE inhibited the expression of PDCD4 protein by increasing the expression of miR-424 in BCEC/TF cells.
In summary, the therapeutic effect of PEG-PLGA/PUE nanoparticles on the in vitro cell model of cerebral infarction is better than that of PUE.
Moreover, PEG-PLGA/PUE inhibits the expression of PDCD4 protein by lowering the expression level of miR-424 in cells, thereby reducing the hazard of cerebral infarction.
American Psychological Association (APA)
Li, Lei& Li, Yan& Miao, Cheng& Liu, Rui. 2020. Improved Therapeutic Effect of Puerarin-Encapsulated PEG-PLGA Nanoparticle on an In Vitro Cerebral Infarction Model. Advances in Polymer Technology،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1130343
Modern Language Association (MLA)
Li, Lei…[et al.]. Improved Therapeutic Effect of Puerarin-Encapsulated PEG-PLGA Nanoparticle on an In Vitro Cerebral Infarction Model. Advances in Polymer Technology No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1130343
American Medical Association (AMA)
Li, Lei& Li, Yan& Miao, Cheng& Liu, Rui. Improved Therapeutic Effect of Puerarin-Encapsulated PEG-PLGA Nanoparticle on an In Vitro Cerebral Infarction Model. Advances in Polymer Technology. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1130343
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130343