Polymethacrylate Sphere-Based Assay for Ultrasensitive miRNA Detection
Joint Authors
Rito-Palomares, Marco
Vázquez-Villegas, Patricia
Dominguez, Margarita S.
Hosseini, Samira
Willson, Richard C.
Koole, Leo H.
Madou, Marc J.
Martínez-Chapa, Sergio O.
Source
Advances in Polymer Technology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-01
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Although microRNAs (miRNAs) have emerged as increasingly important target analytes, their biorecognition remains challenging due to their small size, high sequence homology, and low abundance in clinical samples.
Nanospheres and microspheres have also gained increasing attention in biosensor applications due to their high specific surface area and the wide variety of compositions available.
In this study, chemically designed and synthesized microspheres with active functional groups were used to promote effective miRNA immobilization resulting in better biorecognition.
Upon conjugation with fluorescence-labeled complimentary probes, acylate-based spheres have indirectly detected MiR159, offering significantly enhanced analytical sensitivity, specificity, and accuracy while yielding a considerably low limit of detection (LOD) of 40 picomolar.
Furthermore, MiR159 presence, which is known to be inversely correlated to breast cancer incidence and progression, was successfully detected in a competitive assay, which is promising for upgrading the current assay to clinical use.
American Psychological Association (APA)
Hosseini, Samira& Vázquez-Villegas, Patricia& Willson, Richard C.& Rito-Palomares, Marco& Dominguez, Margarita S.& Koole, Leo H.…[et al.]. 2020. Polymethacrylate Sphere-Based Assay for Ultrasensitive miRNA Detection. Advances in Polymer Technology،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1130351
Modern Language Association (MLA)
Hosseini, Samira…[et al.]. Polymethacrylate Sphere-Based Assay for Ultrasensitive miRNA Detection. Advances in Polymer Technology No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1130351
American Medical Association (AMA)
Hosseini, Samira& Vázquez-Villegas, Patricia& Willson, Richard C.& Rito-Palomares, Marco& Dominguez, Margarita S.& Koole, Leo H.…[et al.]. Polymethacrylate Sphere-Based Assay for Ultrasensitive miRNA Detection. Advances in Polymer Technology. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1130351
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130351