Development of Nanozyme-Labeled Biomimetic Immunoassay for Determination of Sulfadiazine Residue in Foods
Joint Authors
He, Jingbo
Zhang, Li
Xu, Longhua
Kong, Feifan
Xu, Zhixiang
Source
Advances in Polymer Technology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The excessive use and ingestion of residues of sulfonamides harm the human body and the environment.
To meet the requirements of the maximum residue limit specified by the Ministry of Agriculture of China, a rapid detection method urgently needs to be developed.
In the present study, a molecularly imprinted polymer (MIP) that could selectively recognize sulfadiazine (SDZ) was prepared using SDZ as the template molecule and methacrylic acid as the functional monomer.
Using MIP as biomimetic antibody and Au@Pt@SiO2 nanozyme as a marker, a new biomimetic immunoassay was developed to detect sulfadiazine.
Under the optimal conditions, the limit of detection (IC15) and sensitivity (IC50) of this method were 0.09 and 6.1 mg/L, respectively.
To determine the accuracy of this method, honey and milk samples spiked with sulfadiazine were analyzed, with recoveries in the range of 70.8%-90.2%.
The method was also used for the quantitative analysis of sulfadiazine residues in milk powder and milk samples, producing results which correlated well with those obtained by high-performance liquid chromatography.
American Psychological Association (APA)
He, Jingbo& Zhang, Li& Xu, Longhua& Kong, Feifan& Xu, Zhixiang. 2020. Development of Nanozyme-Labeled Biomimetic Immunoassay for Determination of Sulfadiazine Residue in Foods. Advances in Polymer Technology،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1130374
Modern Language Association (MLA)
He, Jingbo…[et al.]. Development of Nanozyme-Labeled Biomimetic Immunoassay for Determination of Sulfadiazine Residue in Foods. Advances in Polymer Technology No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1130374
American Medical Association (AMA)
He, Jingbo& Zhang, Li& Xu, Longhua& Kong, Feifan& Xu, Zhixiang. Development of Nanozyme-Labeled Biomimetic Immunoassay for Determination of Sulfadiazine Residue in Foods. Advances in Polymer Technology. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1130374
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130374