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Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex
Joint Authors
Source
Biochemistry Research International
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Med6 protein (Med6p) is a hallmark component of evolutionarily conserved Mediator complexes, and the genuine role of Med6p in Mediator functions remains elusive.
For the functional analysis of Saccharomyces cerevisiae Med6p (scMed6p), we generated a series of scMed6p mutants harboring a small internal deletion.
Genetic analysis of these mutants revealed that three regions (amino acids 33–42 (Δ2), 125–134 (Δ5), and 157–166 (Δ6)) of scMed6p are required for cell viability and are located at highly conserved regions of Med6 homologs.
Notably, the Med6p-Δ2 mutant was barely detectable in whole-cell extracts and purified Mediator, suggesting a loss of Mediator association and concurrent rapid degradation.
Consistent with this, the recombinant forms of Med6p having these mutations partially (Δ2) restore or fail (Δ5 and Δ6) to restore in vitro transcriptional defects caused by temperature-sensitive med6 mutation.
In an artificial recruitment assay, Mediator containing a LexA-fused wild-type Med6p or Med6p-Δ5 was recruited to the lexA operator region with TBP and activated reporter gene expression.
However, the recruitment of Mediator containing LexA-Med6p-Δ6 to lexA operator region resulted in neither TBP recruitment nor reporter gene expression.
This result demonstrates a pivotal role of Med6p in the postrecruitment function of Mediator, which is essential for transcriptional activation by Mediator.
American Psychological Association (APA)
Kim, Gwang Sik& Lee, Young Chul. 2018. Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex. Biochemistry Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1130391
Modern Language Association (MLA)
Kim, Gwang Sik& Lee, Young Chul. Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex. Biochemistry Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1130391
American Medical Association (AMA)
Kim, Gwang Sik& Lee, Young Chul. Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex. Biochemistry Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1130391
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130391