HBV Core Promoter Inhibition by Tubulin Polymerization Inhibitor (SRI-32007)‎

Joint Authors

Cai, Zhaohui
Kalkeri, Raj
Peng, Junzhong
Huang, Chunsheng
Ptak, Roger G.
Suto, Mark J.

Source

Advances in Virology

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-10-14

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Biology

Abstract EN

Approximately 257 million people chronically infected with hepatitis B virus (HBV) worldwide are at risk of developing hepatocellular carcinoma (HCC).

However, despite the availability of potent nucleoside/tide inhibitors, currently there are no curative therapies for chronic HBV infections.

To identify potential new antiviral molecules, a select group of compounds previously evaluated in clinical studies were tested against 12 different viruses.

Amongst the compounds tested, SRI-32007 (CYT997) demonstrated antiviral activity against HBV (genotype D) in HepG2.2.2.15 cell-based virus yield assay with 50% effective concentration (EC50) and selectivity index (SI) of 60.1 nM and 7.2, respectively.

Anti-HBV activity of SRI-32007 was further confirmed against HBV genotype B in huh7 cells with secreted HBe antigen endpoint (EC50 40 nM and SI 250).

To determine the stage of HBV life cycle inhibited by SRI-32007, time of addition experiment was conducted in HepG2-NTCP cell-based HBV infectious assay.

Results indicated that SRI-32007 retained anti-HBV activity even when added 72 hours postinfection (72 h).

Additional mechanism of action studies demonstrated potent inhibition of HBV core promoter activity by SRI-32007 with an EC50 of 40 nM and SI of >250.

This study demonstrates anti-HBV activity of a repurposed compound SRI-32007 through inhibition of HBV core promoter activity.

Further evaluation of SRI-32007 in HBV animal models is needed to confirm its activity in vivo.

Our experiments illustrate the utility of repurposing strategy to identify novel antiviral chemical leads.

HBV core promoter inhibitors such as SRI-32007 might enable the development of novel therapeutic strategies to combat HBV infections.

American Psychological Association (APA)

Kalkeri, Raj& Peng, Junzhong& Huang, Chunsheng& Cai, Zhaohui& Ptak, Roger G.& Suto, Mark J.. 2020. HBV Core Promoter Inhibition by Tubulin Polymerization Inhibitor (SRI-32007). Advances in Virology،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1130684

Modern Language Association (MLA)

Kalkeri, Raj…[et al.]. HBV Core Promoter Inhibition by Tubulin Polymerization Inhibitor (SRI-32007). Advances in Virology No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1130684

American Medical Association (AMA)

Kalkeri, Raj& Peng, Junzhong& Huang, Chunsheng& Cai, Zhaohui& Ptak, Roger G.& Suto, Mark J.. HBV Core Promoter Inhibition by Tubulin Polymerization Inhibitor (SRI-32007). Advances in Virology. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1130684

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1130684