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High Throughput Approaches to Unravel the Mechanism of Action of a New Vanadium-Based Compound against Trypanosoma cruzi
Joint Authors
Gehrke, S. A.
Mosquillo, M. Florencia
Smircich, Pablo
Lima, Analía
Scalese, Gonzalo
Machado, Ignacio
Gambino, Dinorah
Garat, Beatriz
Pérez-Díaz, Leticia
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-11
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Treatment for Chagas disease, a parasitosis caused by Trypanosoma cruzi, has always been based on two drugs, nifurtimox and benznidazole, despite the toxic side effects described after prolonged prescription.
In this work, we study a new prospective antitrypanosomal drug based on vanadium, here named VIVO(5Brsal)(aminophen).
We found a good IC50 value, (3.76 ± 0.08) μM, on CL Brener epimastigotes.
The analysis of cell death mechanism allowed us to rule out the implication of a mechanism based on early apoptosis or necrosis.
Recovery assays revealed a trypanostatic effect, accompanied by cell shape and motility alterations.
An uptake mostly associated with the insoluble fraction of the parasites was deduced through vanadium determinations.
Concordantly, no drastic changes of the parasite transcriptome were detected after 6 h of treatment.
Instead, proteomic analysis uncovered the modulation of proteins involved in different processes such as energy and redox metabolism, transport systems, detoxifying pathways, ribosomal protein synthesis, and proteasome protein degradation.
Overall, the results here presented lead us to propose that VIVO(5Brsal)(aminophen) exerts a trypanostatic effect on T.
cruzi affecting parasite insoluble proteins.
American Psychological Association (APA)
Mosquillo, M. Florencia& Smircich, Pablo& Lima, Analía& Gehrke, S. A.& Scalese, Gonzalo& Machado, Ignacio…[et al.]. 2020. High Throughput Approaches to Unravel the Mechanism of Action of a New Vanadium-Based Compound against Trypanosoma cruzi. Bioinorganic Chemistry and Applications،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1130703
Modern Language Association (MLA)
Mosquillo, M. Florencia…[et al.]. High Throughput Approaches to Unravel the Mechanism of Action of a New Vanadium-Based Compound against Trypanosoma cruzi. Bioinorganic Chemistry and Applications No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1130703
American Medical Association (AMA)
Mosquillo, M. Florencia& Smircich, Pablo& Lima, Analía& Gehrke, S. A.& Scalese, Gonzalo& Machado, Ignacio…[et al.]. High Throughput Approaches to Unravel the Mechanism of Action of a New Vanadium-Based Compound against Trypanosoma cruzi. Bioinorganic Chemistry and Applications. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1130703
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130703