Finite Element Modelling of Single Cell Based on Atomic Force Microscope Indentation Method
Joint Authors
Chen, Weiyi
Wang, Lili
Wang, Li
Xu, Limeng
Source
Computational and Mathematical Methods in Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The stiffness of cells, especially cancer cells, is a key mechanical property that is closely associated with their biomechanical functions, such as the mechanotransduction and the metastasis mechanisms of cancer cells.
In light of the low survival rate of single cells and measurement uncertainty, the finite element method (FEM) was used to quantify the deformations and predict the stiffness of single cells.
To study the effect of the cell components on overall stiffness, two new FEM models were proposed based on the atomic force microscopy (AFM) indentation method.
The geometric sizes of the FEM models were determined by AFM topography images, and the validity of the FEM models was verified by comparison with experimental data.
The effect of the intermediate filaments (IFs) and material properties of the cellular continuum components on the overall stiffness were investigated.
The experimental results showed that the stiffness of cancer cells has apparent positional differences.
The FEM simulation results show that IFs contribute only slightly to the overall stiffness within 10% strain, although they can transfer forces directly from the membrane to the nucleus.
The cytoskeleton (CSK) is the major mechanical component of a cell.
Furthermore, parameter studies revealed that the material properties (thickness and elasticity) of the continuum have a significant influence on the overall cellular stiffness while Poisson’s ratio has less of an influence on the overall cellular stiffness.
The proposed FEM models can determine the contribution of the major components of the cells to the overall cellular stiffness and provide insights for understanding the response of cells to the external mechanical stimuli and studying the corresponding mechanical mechanisms and cell biomechanics.
American Psychological Association (APA)
Wang, Lili& Wang, Li& Xu, Limeng& Chen, Weiyi. 2019. Finite Element Modelling of Single Cell Based on Atomic Force Microscope Indentation Method. Computational and Mathematical Methods in Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1130719
Modern Language Association (MLA)
Wang, Lili…[et al.]. Finite Element Modelling of Single Cell Based on Atomic Force Microscope Indentation Method. Computational and Mathematical Methods in Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1130719
American Medical Association (AMA)
Wang, Lili& Wang, Li& Xu, Limeng& Chen, Weiyi. Finite Element Modelling of Single Cell Based on Atomic Force Microscope Indentation Method. Computational and Mathematical Methods in Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1130719
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130719