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RASBRAF Circulating Tumor DNA Mutations as a Predictor of Response to First-Line Chemotherapy in Metastatic Colorectal Cancer Patients
Joint Authors
An, Guangyu
Yao, Jiannan
Ge, Yang
Zang, Wanchun
Weygant, Nathaniel
Yu, Pan
Li, Lei
Rao, Guanhua
Jiang, Zhi
Yan, Rui
He, Linjia
Yu, Yang
Jin, Mulan
Cheng, Gang
Source
Canadian Journal of Gastroenterology and Hepatology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Since circulating tumor DNA (ctDNA) offers clear advantages as a minimally invasive method for tumor monitoring compared with tumor tissue, we aimed to evaluate genotyping ctDNA using a next-generation sequencing- (NGS-) based panel to identify the prognostic value of mutation status in metastatic colorectal cancer (mCRC) patients with primary tumor resected and with subsequent lines of treatment in this study.
Methods.
76 mCRC patients treated in Beijing Chao-Yang Hospital from 2011 to 2017 were enrolled.
Genotyping of RAS/BRAF in tumor tissue and ctDNA was determined by ARMS PCR and with a 40-gene panel using NGS, respectively.
Patient clinicopathologic features and RAS/BRAF gene mutation status were evaluated by survival analysis for disease-free survival (DFS) and progression-free survival (PFS).
Results.
Among 76 patients, KRAS distributions were not significantly correlated with any clinicopathologic features.
The concordance between tumor tissue and ctDNA KRAS mutation was 81.25%.
Mutations of RAS/BRAF had no significant impact on DFS after surgery (hazard ratio (HR), 1.205; 95% CI, 0.618 to 2.349; P = 0.5837 ) but prognosticated poorer PFS in subsequent first-line therapy (HR, 3.351; 95% CI, 1.172 to 9.576; P = 0.024 ).
Conclusion.
ctDNA was comparable with tumor tissue for mutation detection.
RAS/BRAF mutations detected in ctDNA predict a worse PFS in mCRC patients with first-line chemotherapy.
Our results provide support for the prognostic value of RAS/BRAF ctDNA mutation detection in mCRC patients.
American Psychological Association (APA)
Yao, Jiannan& Zang, Wanchun& Ge, Yang& Weygant, Nathaniel& Yu, Pan& Li, Lei…[et al.]. 2018. RASBRAF Circulating Tumor DNA Mutations as a Predictor of Response to First-Line Chemotherapy in Metastatic Colorectal Cancer Patients. Canadian Journal of Gastroenterology and Hepatology،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1130911
Modern Language Association (MLA)
Yao, Jiannan…[et al.]. RASBRAF Circulating Tumor DNA Mutations as a Predictor of Response to First-Line Chemotherapy in Metastatic Colorectal Cancer Patients. Canadian Journal of Gastroenterology and Hepatology No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1130911
American Medical Association (AMA)
Yao, Jiannan& Zang, Wanchun& Ge, Yang& Weygant, Nathaniel& Yu, Pan& Li, Lei…[et al.]. RASBRAF Circulating Tumor DNA Mutations as a Predictor of Response to First-Line Chemotherapy in Metastatic Colorectal Cancer Patients. Canadian Journal of Gastroenterology and Hepatology. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1130911
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130911