Gastric Cancer Cell Lines Have Different MYC-Regulated Expression Patterns but Share a Common Core of Altered Genes
Joint Authors
Burbano, Rommel Rodríguez
Assumpção, Paulo Pimentel
Maués, Jersey Heitor da S.
Ribeiro, Helem Ferreira
Pinto, Giovanny R.
Lopes, Luana de Oliveira
Lamarão, Letícia M.
Pessoa, Carla Mariana F.
Moreira-Nunes, Caroline de Fátima Aquino
Carvalho, Raimundo Miranda de
Rey, Juan A.
Source
Canadian Journal of Gastroenterology and Hepatology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-16
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
MYC is an oncogene responsible for excessive cell growth in cancer, enabling transcriptional activation of genes involved in cell cycle regulation, metabolism, and apoptosis, and is usually overexpressed in gastric cancer (GC).
By using siRNA and Next-Generation Sequencing (NGS), we identified MYC-regulated differentially expressed Genes (DEGs) in three Brazilian gastric cancer cell lines representing the histological subtypes of GC (diffuse, intestinal, and metastasis).
The DEGs were picked using Sailfish software, followed by Gene Set Enrichment Analysis (GSEA) and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway analysis using KEGG.
We found 11 significantly enriched gene sets by using enrichment score (ES), False Discovery Rate (FDR), and nominal P-values.
We identified a total of 5.471 DEGs with correlation over (80%).
In diffuse-type and in metastatic GC cell lines, MYC-silencing caused DEGs downregulation, while the intestinal-type GC cells presented overall DEGs upregulation after MYC siRNA depletion.
We were able to detect 11 significant gene sets when comparing our samples to the hallmark collection of gene expression, enriched mostly for the following hallmarks: proliferation, pathway, signaling, metabolic, and DNA damage response.
When we analyzed our DEGs considering KEGG metabolic pathways, we found 12 common branches covering a wide range of biological functions, and three of them were common to all three cell lines: ubiquitin-mediated proteolysis, ribosomes, and system and epithelial cell signaling in Helicobacter pylori infection.
The GC cell lines used in this study share 14 MYC-regulated genes, but their gene expression profile is different for each histological subtype of GC.
Our results present a computational analysis of MYC-related signatures in GC, and we present evidence that GC cell lines representing distinct histological subtypes of this disease have different MYC-regulated expression profiles but share a common core of altered genes.
This is an important step towards the understanding of MYC’s role in gastric carcinogenesis and an indication of probable new drug targets in stomach cancer.
American Psychological Association (APA)
Maués, Jersey Heitor da S.& Ribeiro, Helem Ferreira& Pinto, Giovanny R.& Lopes, Luana de Oliveira& Lamarão, Letícia M.& Pessoa, Carla Mariana F.…[et al.]. 2018. Gastric Cancer Cell Lines Have Different MYC-Regulated Expression Patterns but Share a Common Core of Altered Genes. Canadian Journal of Gastroenterology and Hepatology،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1130971
Modern Language Association (MLA)
Maués, Jersey Heitor da S.…[et al.]. Gastric Cancer Cell Lines Have Different MYC-Regulated Expression Patterns but Share a Common Core of Altered Genes. Canadian Journal of Gastroenterology and Hepatology No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1130971
American Medical Association (AMA)
Maués, Jersey Heitor da S.& Ribeiro, Helem Ferreira& Pinto, Giovanny R.& Lopes, Luana de Oliveira& Lamarão, Letícia M.& Pessoa, Carla Mariana F.…[et al.]. Gastric Cancer Cell Lines Have Different MYC-Regulated Expression Patterns but Share a Common Core of Altered Genes. Canadian Journal of Gastroenterology and Hepatology. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1130971
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130971