N-3 Polyunsaturated Fatty Acids Stimulate Bile Acid Detoxification in Human Cell Models
Joint Authors
Vohl, Marie-Claude
Cieślak, Anna
Trottier, Jocelyn
Verreault, Mélanie
Barbier, Olivier
Milkiewicz, Piotr
Source
Canadian Journal of Gastroenterology and Hepatology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-05
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Cholestasis is characterized by the accumulation of toxic bile acids (BAs) in liver cells.
The present study aimed to evaluate the effects of n-3 polyunsaturated fatty acids (n-3 PUFAs), such as docosahexaenoic (DHA) and eicosapentaenoic (EPA) acids, on BA homeostasis and toxicity in human cell models.
The effects of EPA and/or DHA on the expression of genes involved in the maintenance of BA homeostasis were analyzed in human hepatoma (HepG2) and colon carcinoma (Caco-2) cells, as well as in primary culture of human intestinal (InEpC) and renal (RPTEC) cells.
Extracellular BA species were quantified in culture media using LC-MS/MS.
BA-induced toxicity was evaluated using caspase-3 and flow cytometry assays.
Gene expression analyses of HepG2 cells reveal that n-3 PUFAs reduce the expression of genes involved in BA synthesis (CYP7A1, CYP27A1) and uptake (NTCP), while activating genes encoding metabolic enzymes (SULT2A1) and excretion transporters (MRP2, MRP3).
N-3 PUFAs also generate a less toxic BA pool and prevent the BA-dependent activation of apoptosis in HepG2 cells.
Conclusion.
The present study reveals that n-3 PUFAs stimulate BA detoxification.
American Psychological Association (APA)
Cieślak, Anna& Trottier, Jocelyn& Verreault, Mélanie& Milkiewicz, Piotr& Vohl, Marie-Claude& Barbier, Olivier. 2018. N-3 Polyunsaturated Fatty Acids Stimulate Bile Acid Detoxification in Human Cell Models. Canadian Journal of Gastroenterology and Hepatology،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1130981
Modern Language Association (MLA)
Cieślak, Anna…[et al.]. N-3 Polyunsaturated Fatty Acids Stimulate Bile Acid Detoxification in Human Cell Models. Canadian Journal of Gastroenterology and Hepatology No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1130981
American Medical Association (AMA)
Cieślak, Anna& Trottier, Jocelyn& Verreault, Mélanie& Milkiewicz, Piotr& Vohl, Marie-Claude& Barbier, Olivier. N-3 Polyunsaturated Fatty Acids Stimulate Bile Acid Detoxification in Human Cell Models. Canadian Journal of Gastroenterology and Hepatology. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1130981
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130981