Urinary Elimination of Bile Acid Glucuronides under Severe Cholestatic Situations: Contribution of Hepatic and Renal Glucuronidation Reactions
Joint Authors
Trottier, Jocelyn
Verreault, Mélanie
Perreault, Martin
Białek, Andrzej
Caron, Patrick
Poirier, Guy G.
Wunsch, Ewa
Barbier, Olivier
Milkiewicz, Piotr
Source
Canadian Journal of Gastroenterology and Hepatology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Biliary obstruction, a severe cholestatic complication, causes accumulation of toxic bile acids (BAs) in liver cells.
Glucuronidation, catalyzed by UDP-glucuronosyltransferase (UGT) enzymes, detoxifies cholestatic BAs.
Using liquid chromatography coupled to tandem mass spectrometry, 11 BA glucuronide (-G) species were quantified in prebiliary and postbiliary stenting serum and urine samples from 17 patients with biliary obstruction.
Stenting caused glucuronide- and fluid-specific changes in BA-G levels and BA-G/BA metabolic ratios.
In vitro glucuronidation assays with human liver and kidney microsomes revealed that even if renal enzymes generally displayed lower K M values, the two tissues shared similar glucuronidation capacities for BAs.
By contrast, major differences between the two tissues were observed when four human BA-conjugating UGTs 1A3, 1A4, 2B4, and 2B7 were analyzed for mRNA and protein levels.
Notably, the BA-24G producing UGT1A3 enzyme, abundant in the liver, was not detected in kidney microsomes.
In conclusion, the circulating and urinary BA-G profiles are hugely impacted under severe cholestasis.
The similar BA-glucuronidating abilities of hepatic and renal extracts suggest that both the liver and kidney may contribute to the urine BA-G pool.
American Psychological Association (APA)
Perreault, Martin& Wunsch, Ewa& Białek, Andrzej& Trottier, Jocelyn& Verreault, Mélanie& Caron, Patrick…[et al.]. 2018. Urinary Elimination of Bile Acid Glucuronides under Severe Cholestatic Situations: Contribution of Hepatic and Renal Glucuronidation Reactions. Canadian Journal of Gastroenterology and Hepatology،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1131043
Modern Language Association (MLA)
Perreault, Martin…[et al.]. Urinary Elimination of Bile Acid Glucuronides under Severe Cholestatic Situations: Contribution of Hepatic and Renal Glucuronidation Reactions. Canadian Journal of Gastroenterology and Hepatology No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1131043
American Medical Association (AMA)
Perreault, Martin& Wunsch, Ewa& Białek, Andrzej& Trottier, Jocelyn& Verreault, Mélanie& Caron, Patrick…[et al.]. Urinary Elimination of Bile Acid Glucuronides under Severe Cholestatic Situations: Contribution of Hepatic and Renal Glucuronidation Reactions. Canadian Journal of Gastroenterology and Hepatology. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1131043
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131043