Co(II) Coordination in Prokaryotic Zinc Finger Domains as Revealed by UV-Vis Spectroscopy
Joint Authors
Sivo, Valeria
D’Abrosca, Gianluca
Russo, Luigi
Iacovino, Rosa
Pedone, Paolo Vincenzo
Fattorusso, Roberto
Isernia, Carla
Malgieri, Gaetano
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Co(II) electronic configuration allows its use as a spectroscopic probe in UV-Vis experiments to characterize the metal coordination sphere that is an essential component of the functional structure of zinc-binding proteins and to evaluate the metal ion affinities of these proteins.
Here, exploiting the capability of the prokaryotic zinc finger to use different combinations of residues to properly coordinate the structural metal ion, we provide the UV-Vis characterization of Co(II) addition to Ros87 and its mutant Ros87_C27D which bears an unusual CysAspHis2 coordination sphere.
Zinc finger sites containing only one cysteine have been infrequently characterized.
We show for the CysAspHis2 coordination an intense d-d transition band, blue-shifted with respect to the Cys2His2 sphere.
These data complemented by NMR and CD data demonstrate that the tetrahedral geometry of the metal site is retained also in the case of a single-cysteine coordination sphere.
American Psychological Association (APA)
Sivo, Valeria& D’Abrosca, Gianluca& Russo, Luigi& Iacovino, Rosa& Pedone, Paolo Vincenzo& Fattorusso, Roberto…[et al.]. 2017. Co(II) Coordination in Prokaryotic Zinc Finger Domains as Revealed by UV-Vis Spectroscopy. Bioinorganic Chemistry and Applications،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1131106
Modern Language Association (MLA)
Sivo, Valeria…[et al.]. Co(II) Coordination in Prokaryotic Zinc Finger Domains as Revealed by UV-Vis Spectroscopy. Bioinorganic Chemistry and Applications No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1131106
American Medical Association (AMA)
Sivo, Valeria& D’Abrosca, Gianluca& Russo, Luigi& Iacovino, Rosa& Pedone, Paolo Vincenzo& Fattorusso, Roberto…[et al.]. Co(II) Coordination in Prokaryotic Zinc Finger Domains as Revealed by UV-Vis Spectroscopy. Bioinorganic Chemistry and Applications. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1131106
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131106