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Molecular Characteristics of Methicillin-Resistant Staphylococci Clinical Isolates from a Tertiary Hospital in Northern Thailand
Joint Authors
Kitti, Thawatchai
Seng, Rathanin
Saiprom, Natnaree
Thummeepak, Rapee
Chantratita, Narisara
Boonlao, Chalermchai
Sitthisak, Sutthirat
Source
Canadian Journal of Infectious Diseases and Medical Microbiology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Methicillin-resistant staphylococci are now recognized as a major cause of infectious diseases, particularly in hospitals.
Molecular epidemiology is important for prevention and control of infection, but little information is available regarding staphylococcal infections in Northern Thailand.
In the present study, we examined antimicrobial susceptibility patterns, detection of antimicrobial resistance genes, and SCCmec types of methicillin-resistant S.
aureus (MRSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) isolated from patients in a hospital in Northern Thailand.
The species of MRSA and MR-CoNS were identified using combination methods, including PCR, MALDI-TOF-MS, and tuf gene sequencing.
The susceptibility pattern of all isolates was determined by the disk diffusion method.
Antimicrobial resistance genes, SCCmec types, and ST239 were characterized using single and multiplex PCR.
ST239 was predominant in MRSA isolates (10/23).
All MR-CoNS (N=31) were identified as S.
haemolyticus (N=18), S.
epidermidis (N=3), S.
cohnii (N=3), S.
capitis (N=6), and S.
hominis (N=1).
More than 70% of MRSA and MR-CoNS were resistant to cefoxitin, penicillin, oxacillin, erythromycin, clindamycin, gentamicin, and ciprofloxacin.
In MRSA isolates, the prevalence of ermA (78.3%) and ermB (73.9%) genes was high compared to that of the ermC gene (4.3%).
In contrast, ermC (87.1%) and qacA/B genes (70.9%) were predominant in MR-CoNS isolates.
SCCmec type III was the dominant type of MRSA (13/23), whereas SCCmec type II was more present in S.
haemolyticus (10/18).
Ten MRSA isolates with SCCmec type III were ST239, which is the common type of MRSA in Asia.
This finding provides useful information for a preventive health strategy directed against methicillin-resistant staphylococcal infections.
American Psychological Association (APA)
Kitti, Thawatchai& Seng, Rathanin& Saiprom, Natnaree& Thummeepak, Rapee& Chantratita, Narisara& Boonlao, Chalermchai…[et al.]. 2018. Molecular Characteristics of Methicillin-Resistant Staphylococci Clinical Isolates from a Tertiary Hospital in Northern Thailand. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1131242
Modern Language Association (MLA)
Kitti, Thawatchai…[et al.]. Molecular Characteristics of Methicillin-Resistant Staphylococci Clinical Isolates from a Tertiary Hospital in Northern Thailand. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1131242
American Medical Association (AMA)
Kitti, Thawatchai& Seng, Rathanin& Saiprom, Natnaree& Thummeepak, Rapee& Chantratita, Narisara& Boonlao, Chalermchai…[et al.]. Molecular Characteristics of Methicillin-Resistant Staphylococci Clinical Isolates from a Tertiary Hospital in Northern Thailand. Canadian Journal of Infectious Diseases and Medical Microbiology. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1131242
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131242