Development of Microsatellite Markers for the Nipa Palm Hispid Beetle, Octodonta nipae (Maulik)
Joint Authors
Chen, Zhiming
Chen, Jun
Zhang, Xiang
Hou, Youming
Wang, Guihua
Source
Canadian Journal of Infectious Diseases and Medical Microbiology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-07
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The nipa palm hispid beetle, Octodonta nipae (Maulik) (Coleoptera: Chrysomelidae), is an important invasive pest on palm plants in southern China.
Based on existing transcriptome data, polymorphism simple sequence repeat (SSR) loci were identified.
In total, 1274 SSR loci were identified from 49919 unigenes.
The majority of them contained mononucleotide, dinucleotide, and trinucleotide motifs (43.56%, 26.14%, and 28.18%), in which A/T (41.21%) and AT/TA (15.86%) were the most abundant motifs.
104 pairs of the SSR primers produced amplification bands of expected sizes in O.
nipae, 80 pairs of SSR primers were tested randomly for polymorphism, 9 loci of them were validated to be polymorphic markers, and the number of alleles ranged from 2 to 3, with an average of 2.56 per locus.
The population of Zhangzhou and Fuzhou was analyzed by the 9 loci (On1–On9).
These SSR transcriptome data can provide invaluable resource for SSR development, population genetics research, invasion and expansion mechanism, paternity testing, and other research on O.
nipae and its related species.
American Psychological Association (APA)
Chen, Zhiming& Chen, Jun& Zhang, Xiang& Hou, Youming& Wang, Guihua. 2018. Development of Microsatellite Markers for the Nipa Palm Hispid Beetle, Octodonta nipae (Maulik). Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1131249
Modern Language Association (MLA)
Chen, Zhiming…[et al.]. Development of Microsatellite Markers for the Nipa Palm Hispid Beetle, Octodonta nipae (Maulik). Canadian Journal of Infectious Diseases and Medical Microbiology No. 2018 (2018), pp.1-5.
https://search.emarefa.net/detail/BIM-1131249
American Medical Association (AMA)
Chen, Zhiming& Chen, Jun& Zhang, Xiang& Hou, Youming& Wang, Guihua. Development of Microsatellite Markers for the Nipa Palm Hispid Beetle, Octodonta nipae (Maulik). Canadian Journal of Infectious Diseases and Medical Microbiology. 2018. Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1131249
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131249