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Preclinical In Vitro and In Vivo Evaluation of [18F]FE@SUPPY for Cancer PET Imaging: Limitations of a Xenograft Model for Colorectal Cancer
Joint Authors
Wadsak, W.
Jensen-Jarolim, Erika
Hacker, Marcus
Nics, Lukas
Berroterán-Infante, Neydher
Pallitsch, Katharina
Mitterhauser, Markus
Balber, T.
Singer, J.
Dumanic, M.
Fetty, L.
Fazekas-Singer, J.
Vraka, C.
Bergmann, M.
Spreitzer, H.
Viernstein, H.
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Molecular imaging probes such as PET-tracers have the potential to improve the accuracy of tumor characterization by directly visualizing the biochemical situation.
Thus, molecular changes can be detected early before morphological manifestation.
The A3 adenosine receptor (A3AR) is described to be highly expressed in colon cancer cell lines and human colorectal cancer (CRC), suggesting this receptor as a tumor marker.
The aim of this preclinical study was the evaluation of F18FE@SUPPY as a PET-tracer for CRC using in vitro imaging and in vivo PET imaging.
First, affinity and selectivity of FE@SUPPY and its metabolites were determined, proving the favorable binding profile of FE@SUPPY.
The human adenocarcinoma cell line HT-29 was characterized regarding its hA3AR expression and was subsequently chosen as tumor graft.
Promising results regarding the potential of F18FE@SUPPY as a PET-tracer for CRC imaging were obtained by autoradiography as ≥2.3-fold higher accumulation of F18FE@SUPPY was found in CRC tissue compared to adjacent healthy colon tissue from the same patient.
Nevertheless, first in vivo studies using HT-29 xenografts showed insufficient tumor uptake due to (1) poor conservation of target expression in xenografts and (2) unfavorable pharmacokinetics of F18FE@SUPPY in mice.
We therefore conclude that HT-29 xenografts are not adequate to visualize hA3ARs using F18FE@SUPPY.
American Psychological Association (APA)
Balber, T.& Singer, J.& Berroterán-Infante, Neydher& Dumanic, M.& Fetty, L.& Fazekas-Singer, J.…[et al.]. 2018. Preclinical In Vitro and In Vivo Evaluation of [18F]FE@SUPPY for Cancer PET Imaging: Limitations of a Xenograft Model for Colorectal Cancer. Contrast Media & Molecular Imaging،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1131283
Modern Language Association (MLA)
Balber, T.…[et al.]. Preclinical In Vitro and In Vivo Evaluation of [18F]FE@SUPPY for Cancer PET Imaging: Limitations of a Xenograft Model for Colorectal Cancer. Contrast Media & Molecular Imaging No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1131283
American Medical Association (AMA)
Balber, T.& Singer, J.& Berroterán-Infante, Neydher& Dumanic, M.& Fetty, L.& Fazekas-Singer, J.…[et al.]. Preclinical In Vitro and In Vivo Evaluation of [18F]FE@SUPPY for Cancer PET Imaging: Limitations of a Xenograft Model for Colorectal Cancer. Contrast Media & Molecular Imaging. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1131283
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131283