Spectral Photon-Counting Molecular Imaging for Quantification of Monoclonal Antibody-Conjugated Gold Nanoparticles Targeted to Lymphoma and Breast Cancer: An In Vitro Study
Joint Authors
Moghiseh, Mahdieh
Lowe, Chiara
Lewis, John G.
Kumar, Dhiraj
Butler, Anthony
Anderson, Nigel
Raja, Aamir
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The purpose of the present study was to demonstrate an in vitro proof of principle that spectral photon-counting CT can measure gold-labelled specific antibodies targeted to specific cancer cells.
A crossover study was performed with Raji lymphoma cancer cells and HER2-positive SKBR3 breast cancer cells using a MARS spectral CT scanner.
Raji cells were incubated with monoclonal antibody-labelled gold, rituximab (specific antibody to Raji cells), and trastuzumab (as a control); HER2-positive SKBR3 breast cancer cells were incubated with monoclonal antibody-labelled gold, trastuzumab (specific antibody to HER2-positive cancer cells), and rituximab (as a control).
The calibration vials with multiple concentrations of nonfunctionalised gold nanoparticles were used to calibrate spectral CT.
Spectral imaging results showed that the Raji cells-rituximab-gold and HER2-positive cells-trastuzumab-gold had a quantifiable amount of gold, 5.97 mg and 0.78 mg, respectively.
In contrast, both cell lines incubated with control antibody-labelled gold nanoparticles had less gold attached (1.22 mg and 0.15 mg, respectively).
These results demonstrate the proof of principle that spectral molecular CT imaging can identify and quantify specific monoclonal antibody-labelled gold nanoparticles taken up by Raji cells and HER2-positive SKBR3 breast cancer cells.
The present study reports the future potential of spectral molecular imaging in detecting tumour heterogeneity so that treatment can be tuned accordingly, leading to more effective personalised medicine.
American Psychological Association (APA)
Moghiseh, Mahdieh& Lowe, Chiara& Lewis, John G.& Kumar, Dhiraj& Butler, Anthony& Anderson, Nigel…[et al.]. 2018. Spectral Photon-Counting Molecular Imaging for Quantification of Monoclonal Antibody-Conjugated Gold Nanoparticles Targeted to Lymphoma and Breast Cancer: An In Vitro Study. Contrast Media & Molecular Imaging،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1131307
Modern Language Association (MLA)
Moghiseh, Mahdieh…[et al.]. Spectral Photon-Counting Molecular Imaging for Quantification of Monoclonal Antibody-Conjugated Gold Nanoparticles Targeted to Lymphoma and Breast Cancer: An In Vitro Study. Contrast Media & Molecular Imaging No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1131307
American Medical Association (AMA)
Moghiseh, Mahdieh& Lowe, Chiara& Lewis, John G.& Kumar, Dhiraj& Butler, Anthony& Anderson, Nigel…[et al.]. Spectral Photon-Counting Molecular Imaging for Quantification of Monoclonal Antibody-Conjugated Gold Nanoparticles Targeted to Lymphoma and Breast Cancer: An In Vitro Study. Contrast Media & Molecular Imaging. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1131307
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131307