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Multicolour In Vivo Bioluminescence Imaging Using a NanoLuc‐Based BRET Reporter in Combination with Firefly Luciferase
Joint Authors
Murray, Patricia
Taylor, Arthur
Sharkey, Jack
Plagge, Antonius
Wilm, Bettina
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The ability to track the biodistribution and fate of multiple cell populations administered to rodents has the potential to facilitate the understanding of biological processes in a range of fields including regenerative medicine, oncology, and host/pathogen interactions.
Bioluminescence imaging is an important tool for achieving this goal, but current protocols rely on systems that have poor sensitivity or require spectral decomposition.
Here, we show that a bioluminescence resonance energy transfer reporter (BRET) based on NanoLuc and LSSmOrange in combination with firefly luciferase enables the unambiguous discrimination of two cell populations in vivo with high sensitivity.
We insert each of these reporter genes into cells using lentiviral vectors and demonstrate the ability to monitor the cells’ biodistribution under a wide range of administration conditions, including the venous or arterial route, and in different tissues including the brain, liver, kidneys, and tumours.
Our protocol allows for the imaging of two cell populations in the same imaging session, facilitating the overlay of the signals and the identification of anatomical positions where they colocalise.
Finally, we provide a method for postmortem confirmation of the presence of each cell population in excised organs.
American Psychological Association (APA)
Taylor, Arthur& Sharkey, Jack& Plagge, Antonius& Wilm, Bettina& Murray, Patricia. 2018. Multicolour In Vivo Bioluminescence Imaging Using a NanoLuc‐Based BRET Reporter in Combination with Firefly Luciferase. Contrast Media & Molecular Imaging،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1131337
Modern Language Association (MLA)
Taylor, Arthur…[et al.]. Multicolour In Vivo Bioluminescence Imaging Using a NanoLuc‐Based BRET Reporter in Combination with Firefly Luciferase. Contrast Media & Molecular Imaging No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1131337
American Medical Association (AMA)
Taylor, Arthur& Sharkey, Jack& Plagge, Antonius& Wilm, Bettina& Murray, Patricia. Multicolour In Vivo Bioluminescence Imaging Using a NanoLuc‐Based BRET Reporter in Combination with Firefly Luciferase. Contrast Media & Molecular Imaging. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1131337
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131337