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Identification of Functional Interactome of Gastric Cancer Cells with Helicobacter pylori Outer Membrane Protein HpaA by HPLC-MSMS
Joint Authors
Zhang, Jianzhong
Fan, Ruyue
Han, Xiurui
Xiao, Di
He, Lihua
Gong, Yanan
Sun, Lu
Fan, Dongjie
You, Yuanhai
Wang, Tong
Yan, Xiaomei
Zhang, Maojun
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
HpaA as an outer membrane protein of Helicobacter pylori (H.
pylori) plays a significant role in the adhesion to the human stomach, but the functional relation between HpaA and gastric epithelial cells is still not clear.
To screen the interaction between HpaA and cellular proteins in gastric epithelial cells, the HpaA protein from H.
pylori 26695 fused with a tag (6× His) was expressed and purified successfully, the secondary structure was estimated by the Circular Dichroism (CD) spectrum, and the purified recombinant protein was used to perform the pull-down assays with gastric cancer cell lines (AGS and SGC-7901) lysates, respectively.
The pull-down proteins were identified by high-performance liquid chromatography tandem mass spectrometry system (HPLC-MS/MS).
A total of 9 and 13 proteins related were analyzed from AGS and SGC-7901 cell lysates, respectively.
ANXA2 was considered as putative HpaA functional partner discovered from lysates of both cell lines with high score and coverage.
It is hypothesized that HpaA may be involved in the biological process of regulation of transcription and nucleic acid metabolism during the adhesion of H.
pylori to human gastric epithelial cells, and HpaA-binding proteins also be used as targets for the development of antiadhesion drugs against H.
pylori.
American Psychological Association (APA)
Fan, Ruyue& Han, Xiurui& Xiao, Di& He, Lihua& Gong, Yanan& Sun, Lu…[et al.]. 2020. Identification of Functional Interactome of Gastric Cancer Cells with Helicobacter pylori Outer Membrane Protein HpaA by HPLC-MSMS. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1131410
Modern Language Association (MLA)
Fan, Ruyue…[et al.]. Identification of Functional Interactome of Gastric Cancer Cells with Helicobacter pylori Outer Membrane Protein HpaA by HPLC-MSMS. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1131410
American Medical Association (AMA)
Fan, Ruyue& Han, Xiurui& Xiao, Di& He, Lihua& Gong, Yanan& Sun, Lu…[et al.]. Identification of Functional Interactome of Gastric Cancer Cells with Helicobacter pylori Outer Membrane Protein HpaA by HPLC-MSMS. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1131410
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131410