Preclinical Evaluation of Radioiodinated Hoechst 33258 for Early Prediction of Tumor Response to Treatment of Vascular-Disrupting Agents
Joint Authors
Song, Shaoli
Zhang, Dongjian
Gao, Meng
Yao, Nan
Jiang, Cuihua
Liu, Wei
Li, Tiannv
Huang, Dejian
Yin, Zhiqi
Qiu, Yunliang
Jin, Qiaomei
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This study aimed to explore the use of 131I-Hoechst 33258 (131I-H33258) for early prediction of tumor response to vascular-disrupting agents (VDAs) with combretastatin-A4 phosphate (CA4P) as a representative.
Necrosis avidity of 131I-H33258 was evaluated in mouse models with muscle necrosis and blocking was used to confirm the tracer specificity.
Therapy response was evaluated by 131I-H33258 SPECT/CT imaging 24 h after CA4P therapy in W256 tumor-bearing rats.
Radiotracer uptake in tumors was validated ex vivo using γ-counting, autoradiography, and histopathological staining.
Results showed that 131I-H33258 had predominant necrosis avidity and could specifically bind to necrotic tissue.
SPECT/CT imaging demonstrated that an obvious “hot spot” could be observed in the CA4P-treated tumor.
Ex vivo γ-counting revealed 131I-H33258 uptake in tumors was increased 2.8-fold in rats treated with CA4P relative to rats treated with vehicle.
Autoradiography and corresponding H&E staining suggested that 131I-H33258 was mainly localized in necrotic tumor area and the higher overall uptake in the treated tumors was attributed to the increased necrosis.
These results suggest that 131I-H33258 can be used to image induction of cell necrosis 24 h after CA4P therapy, which support further molecular design of probes based on scaffold H33258 for monitoring of tumor response to VDAs treatment.
American Psychological Association (APA)
Zhang, Dongjian& Gao, Meng& Yao, Nan& Jiang, Cuihua& Liu, Wei& Li, Tiannv…[et al.]. 2018. Preclinical Evaluation of Radioiodinated Hoechst 33258 for Early Prediction of Tumor Response to Treatment of Vascular-Disrupting Agents. Contrast Media & Molecular Imaging،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1131424
Modern Language Association (MLA)
Zhang, Dongjian…[et al.]. Preclinical Evaluation of Radioiodinated Hoechst 33258 for Early Prediction of Tumor Response to Treatment of Vascular-Disrupting Agents. Contrast Media & Molecular Imaging No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1131424
American Medical Association (AMA)
Zhang, Dongjian& Gao, Meng& Yao, Nan& Jiang, Cuihua& Liu, Wei& Li, Tiannv…[et al.]. Preclinical Evaluation of Radioiodinated Hoechst 33258 for Early Prediction of Tumor Response to Treatment of Vascular-Disrupting Agents. Contrast Media & Molecular Imaging. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1131424
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131424