Small RNA Sequencing Reveals Exosomal miRNAs Involved in the Treatment of Asthma by Scorpio and Centipede
Joint Authors
Fang, Hong
Tang, Binqing
Wu, Yingen
Wu, Yuqin
Shi, Kehua
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Asthma is a common respiratory disease with inflammation in the lungs.
Exosomes and microRNAs (miRNAs) play crucial role in inflammation, whereas the role of exosomal miRNA in asthma remains unknown.
Here, we aimed to identify the key exosomal miRNAs and their underlying mechanisms involved in scorpio and centipede (SC) treatment in asthma.
Eighteen mice were randomly divided into three groups: control group, asthma group, and SC treatment group.
Effect of SC was assessed by hematoxylin-eosin staining and real-time PCR.
Exosomes from asthma and SC treatment groups were analyzed by small RNA-seq.
Results revealed SC significantly alleviated the pathogenesis of asthma and suppressed the release of inflammatory cytokines.
A total of 328 exosomal miRNAs were differentially expressed between the exosomes from asthma and SC-treated mice, including 118 up- and 210 downregulated in SC-treated mice.
The altered exosomal miRNAs were primarily involved in the function of transcription, apoptotic process, and cell adhesion; and pathway of calcium, Wnt, and MAPK signaling.
Real-time PCR verified exosomal miR-147 was downregulated, while miR-98-5p and miR-10a-5p were upregulated in SC-treated mice compared to asthma mice.
Moreover, the target genes of miR-147-3p, miR-98-5p, and miR-10a-5p were mainly enriched in Wnt and MAPK inflammatory signaling.
miR-10a-5p promoted the proliferation of mouse lung epithelial cells and downregulated the expression of Nfat5 and Map2k6.
These data suggest SC-induced exosomal miRNAs might mediate the inflammatory signaling and might be involved in the SC treatment in asthma.
The exosomal miRNAs might be promising candidates for the treatment of asthma.
American Psychological Association (APA)
Tang, Binqing& Wu, Yingen& Fang, Hong& Wu, Yuqin& Shi, Kehua. 2020. Small RNA Sequencing Reveals Exosomal miRNAs Involved in the Treatment of Asthma by Scorpio and Centipede. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1131425
Modern Language Association (MLA)
Tang, Binqing…[et al.]. Small RNA Sequencing Reveals Exosomal miRNAs Involved in the Treatment of Asthma by Scorpio and Centipede. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1131425
American Medical Association (AMA)
Tang, Binqing& Wu, Yingen& Fang, Hong& Wu, Yuqin& Shi, Kehua. Small RNA Sequencing Reveals Exosomal miRNAs Involved in the Treatment of Asthma by Scorpio and Centipede. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1131425
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131425