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Exosomal miR-22-3p Derived from Chronic Rhinosinusitis with Nasal Polyps Regulates Vascular Permeability by Targeting VE-Cadherin
Joint Authors
Zhang, Ting
Pei, Yinyin
Zhou, Yong
Yan, Yongbing
You, Bo
Chen, Jing
Zhang, Wei
Yao, Li
Zhang, Jie
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-12
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
The abnormal vascular permeability is associated with the formation of chronic rhinosinusitis with nasal polyps (CRSwNP).
Previously, our study demonstrated that the nasal lavage fluid- (NLF-) derived exosomes from CRSwNP can promote the vascular permeability of human umbilical vein endothelial cells (HUVECs).
miR-22-3p, a specific differentiated miRNA, is reported to regulate microvessels in some diseases.
This study is purposed to explore the impact of exosomal miR-22-3p derived from CRSwNP on vascular permeability and identify the underlying targets.
Methods.
Exosomes were extracted from NLF of 26 CRSwNP patients and 10 control patients.
Quantitative real-time PCR (qRT- PCR) was applied to evaluate the relative level of exosomal miR-22-3p.
The impact of exosomal miR-22-3p on HUVECs was assessed by permeability assays in vitro.
The potential molecular targets of miR-22-3p were investigated by applying such technologies as dual-luciferase reporter assay and western blot.
Results.
miR-22-3p was upregulated in NLF-derived exosomes from CRSwNP.
Exosomal miR-22-3p derived from CRSwNP enhanced the tubule permeability of HUVECs.
Vascular endothelial- (VE-) cadherin (CDH5) was identified as a direct target of miR-22-3p.
miR-22-3p regulated the vascular permeability by targeting VE-cadherin in HUVECs.
Conclusions.
Exosomal miR-22-3p derived from NLF of CRSwNP plays an important role in regulating vascular permeability by targeting VE-cadherin.
American Psychological Association (APA)
Zhang, Wei& Zhang, Ting& Yan, Yongbing& Zhang, Jie& Zhou, Yong& Pei, Yinyin…[et al.]. 2020. Exosomal miR-22-3p Derived from Chronic Rhinosinusitis with Nasal Polyps Regulates Vascular Permeability by Targeting VE-Cadherin. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1131476
Modern Language Association (MLA)
Zhang, Wei…[et al.]. Exosomal miR-22-3p Derived from Chronic Rhinosinusitis with Nasal Polyps Regulates Vascular Permeability by Targeting VE-Cadherin. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1131476
American Medical Association (AMA)
Zhang, Wei& Zhang, Ting& Yan, Yongbing& Zhang, Jie& Zhou, Yong& Pei, Yinyin…[et al.]. Exosomal miR-22-3p Derived from Chronic Rhinosinusitis with Nasal Polyps Regulates Vascular Permeability by Targeting VE-Cadherin. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1131476
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131476