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111In-DANBIRT In Vivo Molecular Imaging of Inflammatory Cells in Atherosclerosis
Joint Authors
Mota, Roberto
Campen, Matthew J.
Cuellar, Matthew E.
Garver, William S.
Hesterman, Jacob
Qutaish, Mohammed
Daniels, Tamara
Nysus, Monique
Wagner, Carston R.
Norenberg, Jeffrey P.
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Atherosclerosis-related morbidity and mortality remain a global concern.
Atherosclerotic disease follows a slow and silent progression, and the transition from early-stage lesions to vulnerable plaques remains difficult to diagnose.
Inflammation is a key component of the development of atherosclerotic plaque and consequent life-threatening complications.
This study assessed 111In-DANBIRT as an in vivo, noninvasive SPECT/CT imaging probe targeting an inflammatory marker, Lymphocyte Function Associated Antigen-1 (LFA-1), in atherosclerotic plaques.
Methods.
Selective binding of 111In-DANBIRT was assessed using Sprague-Dawley rats exposed to filtered air and ozone (1 ppm) by inhalation for 4 hours to induce a circulating leukocytosis and neutrophilia in peripheral blood.
After 24 hours, whole blood was collected and incubated with radiolabeled DANBIRT (68Ga-DANBIRT and 111In-DANBIRT).
Isolated cell component smeared slides using cytospin technique were stained with Wright-Giemsa stain.
Apolipoprotein E-deficient (apoE−/−) mice were fed either a normal diet or a high-fat diet (HFD) for 8 weeks.
Longitudinal SPECT/CT imaging was performed 3 hours after administration at baseline, 4, and 8 weeks of HFD diet, followed by tissue harvesting for biodistribution, serum lipid analysis, and histology.
3D autoradiography was performed in both groups 24 hours after administration of 111In-DANBIRT.
Results.
Increased specific uptake of radiolabeled DANBIRT by neutrophils in the ozone-exposed group was evidenced by the acute immune response due to 4-hour ozone exposure.
Molecular imaging performed at 3 hours using SPECT/CT imaging evidenced an exponential longitudinal increase in 111In-DANBIRT uptake in atherosclerosis lesions in HFD-fed mice compared to normal-diet-fed mice.
Such results were consistent with increased immune response to vascular injury in cardiovascular and also immune tissues, correlated by 24 hours after administration of 3D autoradiography.
Histologic analysis confirmed atherosclerotic disease progression with an increased vascular lesion area in HFD-fed mice compared to normal-diet-fed mice.
Conclusion.
111In-DANBIRT is a promising molecular imaging probe to assess inflammation in evolving atheroma and atherosclerotic plaque.
American Psychological Association (APA)
Mota, Roberto& Campen, Matthew J.& Cuellar, Matthew E.& Garver, William S.& Hesterman, Jacob& Qutaish, Mohammed…[et al.]. 2018. 111In-DANBIRT In Vivo Molecular Imaging of Inflammatory Cells in Atherosclerosis. Contrast Media & Molecular Imaging،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1131498
Modern Language Association (MLA)
Mota, Roberto…[et al.]. 111In-DANBIRT In Vivo Molecular Imaging of Inflammatory Cells in Atherosclerosis. Contrast Media & Molecular Imaging No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1131498
American Medical Association (AMA)
Mota, Roberto& Campen, Matthew J.& Cuellar, Matthew E.& Garver, William S.& Hesterman, Jacob& Qutaish, Mohammed…[et al.]. 111In-DANBIRT In Vivo Molecular Imaging of Inflammatory Cells in Atherosclerosis. Contrast Media & Molecular Imaging. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1131498
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131498