Preparation of 68Ga-PSMA-11 with a Synthesis Module for Micro PET-CT Imaging of PSMA Expression during Prostate Cancer Progression
Joint Authors
Jia, Ruipeng
Shao, Guoqiang
Wang, Yuebing
Wang, Feng
Wang, Zizheng
Wu, Jianping
Cui, Can
Zang, Shimin
Qiu, Fan
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
To synthesize 68Ga-Glu-urea-Lys(Ahx)-HBED-CC (68Ga-PSMA-11) with a synthesis module and investigate PET-CT imaging to monitor PSMA expression during prostate cancer (PCa) progression and tumor growth in mice bearing subcutaneous PCa xenografts.
Method.
The radiochemical purity and stability of 68Ga-PSMA-11 were determined via radio-HPLC.
The PCa cell lines of different PSMA expression levels (PC3, VCAP±, CWR22RV1+, and LNCaP++) were selected to mimic the PCa progression.
68Ga-PSMA-11 biodistribution was studied by dissection method and in vivo imaging with micro PET-CT.
The expression levels of PSMA in tumor cells and tissues were analyzed by immunofluorescence, flow cytometry, and western blot.
The correlation between PSMA expression and radio-uptake was also evaluated.
2-PMPA preadministration served as a block group.
Results.
The radiochemical purity of 68Ga-PSMA-11 was 99.6 ± 0.1% and stable in vitro for 2 h.
The equilibrium binding constant (Kd) of 68Ga-PSMA-11 to LNCaP, CWR22Rv1, PC-3, and VCAP cells was 4.3 ± 0.8 nM, 16.4 ± 1.3 nM, 225.3 ± 20.8 nM, and 125.6 ± 13.1 nM, respectively.
Results of tumor uptake (% ID and % ID/g or % ID/cm3) of 68Ga-PSMA-11 in biodistribution and micro PET imaging were LNCaP > CWR22RV1 > PC-3 and VCAP due to different PSMA expression levels.
It was confirmed by flow cytometry, western blot, and immunofluorescence.
Tumor uptake (% ID/cm3) of 68Ga-PSMA-11 increased with the tumor anatomical volume in quadratic polynomial fashion and reached the peak (when tumor volume was 0.5 cm3) earlier than tumor uptake (% ID).
Tumor uptake (% ID/cm3) of 68Ga-PSMA-11 based on functional volume correlated well with the PSMA expression in a linear manner (y=9.35x+2.59, R2=0.8924, and p<0.0001); however, low dose 2-PMPA causes rapid renal clearance of increased tumor/kidney uptake of 68Ga-PSMA-11.
Conclusions.
The 68Ga-PSMA-11 PET-CT imaging could invasively evaluate PSMA expression during PCa progression and tumor growth with % ID/cm3 (based on functional volume) as an important index.
Low dose 2-PMPA preadministration might be a choice to decrease kidney uptake of 68Ga-PSMA-11.
American Psychological Association (APA)
Wang, Yuebing& Shao, Guoqiang& Wu, Jianping& Cui, Can& Zang, Shimin& Qiu, Fan…[et al.]. 2018. Preparation of 68Ga-PSMA-11 with a Synthesis Module for Micro PET-CT Imaging of PSMA Expression during Prostate Cancer Progression. Contrast Media & Molecular Imaging،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1131555
Modern Language Association (MLA)
Wang, Yuebing…[et al.]. Preparation of 68Ga-PSMA-11 with a Synthesis Module for Micro PET-CT Imaging of PSMA Expression during Prostate Cancer Progression. Contrast Media & Molecular Imaging No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1131555
American Medical Association (AMA)
Wang, Yuebing& Shao, Guoqiang& Wu, Jianping& Cui, Can& Zang, Shimin& Qiu, Fan…[et al.]. Preparation of 68Ga-PSMA-11 with a Synthesis Module for Micro PET-CT Imaging of PSMA Expression during Prostate Cancer Progression. Contrast Media & Molecular Imaging. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1131555
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131555